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本文检测了慢性阻塞性肺疾病(COPD)急性发作期患者痰及血清IL-17的水平,以探讨它们在COPD气道炎症中的作用。1对象和方法1.1检测对象(1)COPD组23例,男23例,女10例,年龄48～75岁,平均年龄59.5岁,均为COPD急性发作期住院病人,符合中华医学会呼吸学分会制定的COPD诊断标准。正常对照组21例,男15例,女6例,年龄41～69岁,平均年龄56岁,均为健康成年人。1.2痰及血清标本的收集和处理上述实验对照用3.5%的高渗盐水雾化后留取痰液2ml,加入等量10%二硫苏糖醇,振荡后混匀,37℃孵化15min,2000r/min,离心5min,取上清液1ml存处-40℃冰箱中待测。清晨采集空腹静脉血
This article examined sputum and serum IL-17 levels in patients with acute exacerbation of chronic obstructive pulmonary disease (COPD) to investigate their role in COPD airway inflammation. 1 Subjects and methods 1.1 Detection of objects (1) COPD group of 23 patients, 23 males and 10 females, aged 48 to 75 years, mean age 59.5 years, were COPD acute exacerbation inpatients, in line with the Respiratory Society of Chinese Medical Association The development of diagnostic criteria for COPD. 21 cases of normal control group, 15 males and 6 females, aged 41 to 69 years old, average age 56 years old, are healthy adults. 1.2 Collection and treatment of sputum and serum specimens The above experimental control with 3.5% hypertonic saline spray after leaving 2ml sputum, adding the same amount of 10% dithiothreitol, shaking after mixing, 37 ℃ incubation 15min, 2000r / min, centrifuged 5min, take the supernatant 1ml stored at -40 ℃ to be measured in the refrigerator. Early morning fasting venous blood collection