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[目的]观察热疗联合阿霉素对人多发性骨髓瘤细胞株ARH-77的体外增殖抑制、凋亡及Bcl-2表达的影响。[方法]采用MTT法确定阿霉素的工作浓度,以该浓度进行化疗或与热疗的联合,选择温度40℃、41℃及42℃,作用于ARH-77细胞。采用绘制肿瘤细胞生长曲线、MTT法检测对肿瘤细胞增殖的抑制作用;流式细胞仪检测肿瘤细胞凋亡及Bcl-2表达。[结果]阿霉素IC50为8.16μg/ml,以此为实验的工作浓度。单纯热疗组60min对ARH-77细胞有抑制作用(P<0.01),并随温度增高而增强。单纯阿霉素化疗组对ARH-77细胞也有抑制作用。各热化疗组对ARH-77均有明显的抑制作用(P<0.01)。热疗组、化疗组及热化疗组的细胞凋亡率均较对照组显著升高,各组之间差异有统计学意义(P<0.01);Bcl-2蛋白表达下降,各组之间也有显著性差异(P<0.01)。[结论]热疗联合阿霉素能增强对ARH-77细胞的体外抑制作用,提高肿瘤细胞的凋亡率,下调Bcl-2蛋白的表达,为热化疗的临床应用提供实验室依据。
[Objective] To observe the effects of hyperthermia combined with doxorubicin on the proliferation inhibition, apoptosis and Bcl-2 expression of human multiple myeloma cell line ARH-77 in vitro. [Method] The working concentration of doxorubicin was determined by MTT method. Chemotherapy or combination with hyperthermia was performed at this concentration. The cells were treated with ARH-77 cells at the temperatures of 40 ℃, 41 ℃ and 42 ℃. The growth curve of tumor cells was drawn, the inhibition of tumor cell proliferation was detected by MTT assay, and the apoptosis of tumor cells and the expression of Bcl-2 were detected by flow cytometry. [Result] The doxorubicin IC50 was 8.16μg / ml, which is the working concentration of the experiment. The treatment of ARH-77 alone for 60 min in the hyperthermic hyperthermia group was inhibited (P <0.01), and increased with increasing temperature. Adriamycin alone chemotherapy group also inhibited ARH-77 cells. Each thermo-chemotherapy group had obvious inhibitory effect on ARH-77 (P <0.01). The apoptosis rates in hyperthermia, chemotherapy and thermochemotherapy groups were significantly higher than those in control group (P <0.01), while the expression of Bcl-2 protein decreased Significant difference (P <0.01). [Conclusion] Hyperthermia combined with doxorubicin can enhance the inhibitory effect on ARH-77 cells in vitro, increase the apoptosis rate of tumor cells and down-regulate the expression of Bcl-2 protein, providing a laboratory basis for the clinical application of thermochemotherapy.