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目的 研究 β 七叶皂苷对大鼠脑缺血 再灌注损伤的保护作用。 方法 线栓法制备大鼠大脑中动脉阻断(middlecerebralarteryocclusion ,MCAO)短暂局灶性脑缺血模型 ,缺血前给予 β 七叶皂苷钠 1 5 ,30 ,6 0mg·kg- 1 ,po ,7d ,末次给药 1h后制备MCAO模型 ,缺血 2h ,再灌注 2 4h ,评价神经功能状态、脑水肿和脑梗死范围 ,测定大脑缺血区及非缺血区皮层及海马中SOD ,GSH Px ,CAT和Na+ K+ ATPase的活性及MDA的含量。结果 β 七叶皂苷钠能显著改善脑缺血 再灌注后脑梗死体积 ,减轻脑水肿 ,改善神经功能症状 ,与模型组比较 ,具有显著性差异 (P <0 0 1 ) ;同时 β 七叶皂苷钠组的大鼠MCAO再灌注后 ,其脑内SOD ,GSH Px及ATPase的活性明显高于模型组 ,而MDA的含量明显低于模型组 ,均具显著性差异 (P <0 0 1或P <0 0 5 )。结论 β 七叶皂苷钠对大鼠局灶性脑缺血 再灌注损伤具有明显保护作用。
Objective To study the protective effect of β-aescin on cerebral ischemia reperfusion injury in rats. METHODS: A transient cerebral ischemic model of middle cerebral artery occlusion (MCAO) was prepared by thread embolism. Before ischemia, β-aescin sodium was administered 15, 30, 60 mg·kg-1, po, 7d. MCAO model was prepared 1 h after the last administration, 2h ischemia, and 24 h reperfusion. Neurological status, cerebral edema and cerebral infarct range were evaluated. SOD, GSH Px in cerebral cortex and hippocampus of ischemic and non-ischemic regions were measured. CAT and Na+ K+ ATPase activity and MDA content. Results β-aescinate can significantly improve the cerebral infarct volume, reduce cerebral edema and improve neurological symptoms after cerebral ischemia-reperfusion. Compared with the model group, the β-esobarin sodium has significant difference (P <0 01); After reperfusion of MCAO in rats, the activities of SOD, GSH Px and ATPase in the brain were significantly higher than those in the model group, while the content of MDA was significantly lower in the model group than in the model group (P <0 0 1 or P < 0 0 5 ). Conclusion Sodium aescinate has a protective effect on focal cerebral ischemia-reperfusion injury in rats.