目的：动态观察实验大鼠肝癌发生的启动、促进、发展和癌形成的不同阶段中ｐ１６和Ｒｂ基因的表达状态及其与病理改变及γＧＴ酶异常灶变化的关系。方法：采用ＳｏｌｔＦａｒｂｅｒ模型和免疫组化方法。结果：ｐ１６和Ｒｂ基因从促癌阶段的增生结节至癌形成阶段的肝癌组织均表达异常，与ＧＴ酶的表达有一定的吻合性。结论：ｐ１６和Ｒｂ基因的表达异常与肝细胞癌变关系密切。两者在肝癌发生的早期即发生改变，且有相互协同的作用。 OBJECTIVE: To dynamically observe the expression status of p16 and Rb genes at different stages of initiation, promotion, development, and carcinogenesis in experimental rats, and their relationship with pathological changes and changes in γ-GT enzyme abnormalities. Methods: The Solt-Farber model and immunohistochemistry method were used. RESULTS: The expression of p16 and Rb genes was abnormal from the proliferating nodules at the stage of promoting cancer to the tissues of hepatocellular carcinoma at the stage of cancer formation, which was consistent with the expression of GT enzyme. Conclusion: Abnormal expression of p16 and Rb genes is closely related to hepatocellular carcinogenesis. Both of them change during the early stage of liver cancer and have synergistic effects.