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目的:本文研究了同时测定黄曲霉毒素B1、B2、G1和G2(AFB1、AFB2、AFG1、AFG2)的方法。方法:使用免疫亲和柱富集净化,以每升流动相含有0.120 g溴化钾和350μl 4 mol/L硝酸的水:甲醇:乙腈(6:3:2)为洗脱液,高效液相色谱电化学柱后衍生,荧光法测定。结果:方法的选择性较好,线性关系(r>0.999),线性范围:AFB1为0.07μg/kg~9.08μg/kg;AFB2为0.02μg/kg~2.70μg/kg;AFG1为0.07μg/kg~9.0μg/kg;AFG2为0.03μg/kg~2.79μg/kg,相对标准偏差0.5%~3.5%之间,检测限和定量限值(μg/kg):AFB1为0.02,0.07;AFB2为,0.01,0.02;AFG1为0.02,0.07;AFG2为0.01,0.03,回收率为80%~105%。结论:该方法快速、准确,能同时测定AFB1、AFB2、AFG1、AFG2
OBJECTIVE: To study the simultaneous determination of aflatoxins B1, B2, G1 and G2 (AFB1, AFB2, AFG1, AFG2). Methods: Purification by immunoaffinity column was as follows. The mobile phase consisted of 0.120 g potassium bromide and 350 μl 4 mol / L nitric acid in water: methanol: acetonitrile (6: 3: 2) Electrochromatography after column derivatization, determination by fluorescence method. Results: The selectivity of the method was linear (r> 0.999) with a linear range of 0.07 μg / kg to 9.08 μg / kg for AFB1, 0.02 μg / kg to 2.70 μg / kg for AFB2 and 0.07 μg / kg for AFB2 ~ 9.0μg / kg; AFG2 was 0.03μg / kg ~ 2.79μg / kg, the relative standard deviations were 0.5% ~ 3.5%. The limits of detection and quantitation were μg / kg; AFB1 was 0.02,0.07; 0.01,0.02; AFG1 was 0.02,0.07; AFG2 was 0.01,0.03, recovery was 80% ~ 105%. Conclusion: The method is rapid and accurate and can simultaneously determine AFB1, AFB2, AFG1, AFG2