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目的探讨P16~(INK4a)和Ki67在宫颈上皮内瘤变(CIN)组织中阳性表达的意义及病理诊断价值。方法选取经病理诊断为CIN的宫颈组织石蜡包埋标本62例,其中CINⅠ级22例,CINⅡ级18例,CINⅢ级22例,采用SP法分别进行P16~(INK4a)和Ki67的免疫组化染色并检测其表达。结果 P16~(INK4a)和Ki67在CINⅠ~Ⅲ级组织中的阳性表达率均逐渐升高,差异均有统计学意义(P<0.01),P16~(INK4a),Ki67的阳性表达率与CIN病变级别呈正相关(r_1=0.658,r_2=0.604,P<0.01)。结论 P16~(INK4a)和Ki67可以作为CIN分级诊断的辅助指标,有利于提高诊断的准确性和一致性,为临床合理治疗提供有效信息。
Objective To investigate the significance of the positive expression of P16 INK4a and Ki67 in cervical intraepithelial neoplasia (CIN) and the value of pathological diagnosis. Methods Sixty-two cases of cervical tissue paraffin-embedded specimens of pathological diagnosis of CIN were selected, including 22 cases of CINⅠgrade, 18 cases of CINⅡgrade and 22 cases of CINⅢ. Immunohistochemical staining of P16 INK4a and Ki67 were performed by SP method. And detect its expression. Results The positive rates of P16 INK4a and Ki67 in CIN Ⅰ ~ Ⅲ tissues were significantly higher than those in controls (P <0.01). The positive rates of P16 INK4a and Ki67 were significantly correlated with CIN (R_1 = 0.658, r_2 = 0.604, P <0.01). Conclusions P16 INK4a and Ki67 can be used as auxiliary indexes for the diagnosis of CIN, which is helpful to improve the accuracy and consistency of diagnosis and provide effective information for clinical reasonable treatment.