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目的研究分离自内蒙古不同鼠疫疫源地、不同宿主和媒介的鼠疫耶尔森菌的差异区段基因型分布特征。方法选取内蒙古3个鼠疫疫源地分离的鼠疫耶尔森菌307株,采用差异区段分析法,设计24对引物进行DFR位点扩增分型,用Bio Numerics软件分析。结果通过差异区段分型方法共发现13种基因型,其中5种基因型(Gnm1~Gnm5)为本研究首次报道。长爪沙鼠疫源地有9种基因型,主要基因型为G11(71.8%,122/170);达乌尔黄鼠疫源地有6种基因型,主要为G10(44.1%,41/93)和G11(35.5%,33/93);布氏田鼠疫源地有4种基因型,主要为G14(90.9%,40/44)。3个疫源地均分布有G11型。结论内蒙古鼠疫耶尔森菌差异区段基因型分布具有明显的地理分布特征,对分析内蒙古鼠疫的传播和遗传演变具有重要的分子流行病学意义。
Objective To study the distribution characteristics of different genotypes of Yersinia pestis isolates from different plague origin, different hosts and vectors in Inner Mongolia. Methods 307 Yersinia pestis isolates from three plague origin in Inner Mongolia were selected and 24 pairs of primers were designed to amplify DFR loci and analyzed by Bio Numerics software. Results Thirteen genotypes were found by differential genotyping. Five genotypes (Gnm1 ~ Gnm5) were the first reported in this study. There were 9 genotypes in the germplasm resources of Gazelle. The main genotype was G11 (71.8%, 122/170). There were 6 genotypes of Gallium dauricus, mainly G10 (44.1%, 41/93) And G11 (35.5%, 33/93) respectively. There were four genotypes of Brandt’s voles (G9) (90.9%, 40/44). Three foci were distributed with G11 type. Conclusion The distribution of genotypes of Yersinia pestis isolates in Inner Mongolia shows obvious geographical distribution, which is of important molecular epidemiological significance for the analysis of the spread and genetic evolution of plague in Inner Mongolia.