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目的 :探讨非食管癌高发区病例的食管癌组织中聚合酶 β基因的突变情况。 方法 :利用RT PCR、SSCP及DNA序列分析的方法 ,对河南省非食管癌高发区 2 5例食管癌及其癌旁组织中聚合酶 β基因的变异情况进行分析。结果 :2 5例食管癌组织标本中 ,5例SSCP结果异常 ,序列分析显示 ,1例 6 13位A→T ,导致氨基酸序列 16 7位Lys→Ile ;2例 6 6 0位A→G ,导致第 183位Arg→Gly ;2例 6 70位A→G ,导致 186位Glu→Gly ;而对应的癌旁组织标本SSCP结果均无异常。结论 :非高发区食管癌组织中有 polβ突变存在。
Objective: To investigate the mutation of polymerase β gene in esophageal cancer tissues in high incidence of non-esophageal cancer. Methods: The variations of polymerase β gene in 25 cases of esophageal cancer and adjacent tissues of non-esophageal cancer in Henan Province were analyzed by RT-PCR, SSCP and DNA sequence analysis. Results: Five of the 25 cases of esophageal cancer tissues showed abnormal results of SSCP. Sequence analysis showed that 1 case of 6 13 A → T resulted in the amino acid sequence of 16 7 Lys → Ile; 2 cases of 6 6 0 A → G, Resulting in Arg → Gly at position 183; Glu → Gly at position 6 70 → A at position 6 70, while SSCP of corresponding paracancerous tissues showed no abnormality. Conclusion: There is polβ mutation in esophageal carcinoma of non-high incidence area.