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目的 探讨云南省鼠疫菌质粒分子流行病学意义。方法 按Kado和Liu法提取云南省 44个县市及中缅边境一带共 10 2 0株鼠疫菌的质粒 ,经琼脂糖凝胶电泳进行检测分析。结果 云南省鼠疫菌具有相对分子质量 (Mr)分别约 3.93、6 .0 5、2 2 .97、35 .6 5、45 .35、6 4.82、74.5 9、111.36和12 9.5 5× 10 6的 9种质粒 ,其中 3.93、35 .6 5和 111.36× 10 6的 3种质粒为云南省鼠疫菌特有。按质粒组成可分为 10种不同类型的质粒谱 ,其中Ⅰ～Ⅴ种类型较为常见 ,且在分布上具有明显的地区集中现象。结论 根据鼠疫菌质粒特征 ,可将云南省自 1982年以来发生鼠疫流行的地区划分为多片相对独立的疫源地。认为 80年代重新开始的家鼠鼠疫流行 ,可能来源于多片潜伏的鼠疫自然疫源地的复燃。
Objective To investigate the molecular epidemiological significance of plague particles in Yunnan Province. Methods A total of 102 strains of Yersinia pestis isolates from 44 counties in Yunnan Province and between China and Myanmar were extracted by Kado and Liu methods and detected by agarose gel electrophoresis. Results Yersinia pestis possessed the relative molecular mass (Mr) of about 3.93, 6.05, 22.97, 35.65, 45.35, 4.82, 74.5, 9, 111.36 and 12.55 × 10 6 9 kinds of plasmids, of which 3.93, 35.65 and 111.36 × 10 6 three kinds of plasmids are endemic to Yunnan Province. According to the plasmid composition can be divided into 10 different types of plasmid spectrum, of which Ⅰ ~ Ⅴ types are more common, and in the distribution of significant regional concentration. Conclusion According to the plague characteristics of Yersinia pestis, the epidemic areas of plague in Yunnan since 1982 can be divided into several relatively independent foci. It is thought that the resurgence of the plague in the 1980s may be due to the resurgence of multiple latent plague natural foci.