AGB (aboveground fresh biomass) is one of the most important parameters of the crop condition monitored with remote sensing. Hyper spectrum remote sensing with the fine spectrum information becomes the efficient method estimating the vegetation AGB. The research was conducted in Xinjiang, the largest cotton planting region of China. The paper analyzed the correlation between the cotton AGB and reflective spectrum and the first derivative spectrum, and the variation coefficient of the waveband reflectance. According to the analysis above, all of 23 parameters, including the hyper spectrum reflectance, the first derivative spectrum parameters and normalization vegetation indexes, were established. And then the estimation models on cotton AGB of relaxing and compact canopy type were established and tested respectively. The tested results showed that F901, [901,502], [901,629], [901,672] among the reflective spectral parameters and D525, D956, D1019, D1751 among the first derivative spectral parameters had the homogenous effect on different cotton canopy types, and the determination coefficients of the models above all arrive at the significant level of 0.99 confidence interval. At last, the tested results of the homogeneity models for different canopy types indicated the parameters of [901, 502], [901,629], [901,672] have more satisfying veracity than others, and the relative errors are as low as 17.0, 16.3 and 16.7% correspondingly; in contrast, the estimation veracity of the first derivative spectrum parameters of single waveband is low.