论文部分内容阅读
目的分离纯化链霉菌I08A 1776发酵液中的水溶性抗耐药结核活性成分。方法利用离子交换、反相和亲水作用等多种色谱技术进行分离纯化,通过现代波谱学方法鉴定活性成分结构。结果分离得到3个水溶性活性成分,其结构分别确定为链丝菌素E(1)、Nβ-乙酰链丝菌素E(2)和Nβ-乙酰链丝菌素D(3)。链丝菌素E(1)对结核分枝杆菌H37Rv和临床分离耐药株的MIC值为0.25~1.0μg/mL。结论首次利用亲水作用色谱分离纯化微生物发酵液中的水溶性活性化合物。亲水作用色谱在微生物水溶性次级代谢产物的分离纯化中具有广阔的应用前景。链丝菌素E具有较强的体外抗耐药结核分枝杆菌活性。
OBJECTIVE To isolate and purify water-soluble anti-TB drug in Streptomyces I08A 1776 fermentation broth. Methods The compounds were separated and purified by a variety of chromatographic techniques, such as ion exchange, reversed phase and hydrophilic interaction. The structures of active components were identified by modern spectroscopy. Results Three water-soluble active ingredients were isolated and their structures were identified as streptozotocin E (1), Nβ-acetyl streptozotocin E (2) and Nβ-acetyl streptozotocin D (3). The MIC value of streptozotocin E (1) against Mycobacterium tuberculosis H37Rv and clinically isolated drug-resistant strains was 0.25-1.0 μg / mL. Conclusion For the first time, water-soluble active compounds in microbial fermentation broth were separated and purified by hydrophilic interaction chromatography. Hydrophilic interaction chromatography has broad application prospects in the separation and purification of water-soluble secondary metabolites. Streptozotocin E has a strong anti-drug resistant Mycobacterium tuberculosis activity in vitro.