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为了对福建省发生的番茄黄化曲叶病毒病的病原进行分子鉴定,并对其病原分子的变异和进化情况进行分析。根据已知的番茄黄化曲叶病毒(TYLCV)基因组序列设计特异引物进行PCR扩增,对获得的特异性片段回收、克隆并测序,结果表明,PCR分子标记反应扩增到541bp的特异性条带,将该特异性产物经回收和纯化并进行克隆与测序,测序结果经BLAST比对后,表明该分离物的序列与国内外的病毒序列相似度为97%~99%。试验结果表明福建省的5个地市的番茄均已被番茄黄化曲叶病毒病危害,应当加强防治。
In order to molecularly identify the pathogen of tomato yellow leaf curl virus disease in Fujian Province, the variation and evolution of the pathogen molecular were analyzed. Specific primers were designed for PCR amplification based on the known TYLCV genome sequence. The specific fragments obtained were recovered, cloned and sequenced. The results showed that PCR-based molecular tag reaction amplified 541bp specific fragment The specific product was recovered and purified, cloned and sequenced. The results of BLAST analysis showed that the sequence of the isolate was 97% ~ 99% similar to the domestic and foreign virus sequences. The test results show that tomato in five cities of Fujian Province has been endangered by tomato yellow leaf curl virus disease and prevention and control should be strengthened.