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在获得MaTPS2基因全长序列的基础上,采用同源序列比对、聚类分析、实时定量PCR等技术初步探讨香蕉MaTPS2基因的功能。从香蕉A基因组中获得一条3 277bp MaTPS2全长序列,包含一个完整的开放阅读框1 344bp,编码蛋白含447个氨基酸。其氨基酸理化性质分析表明,MaTPS2蛋白属于不稳定疏水性蛋白,具有一个结构域GT1-TPS。通过与已知植物的TPS氨基酸同源序列比对,同源性达77.95%。系统发育树进化关系分析表明,MaTPS2编码的氨基酸序列与银杏TPS氨基酸序列(AAX16014.1)邻近,说明二者的亲缘关系较近。器官特异性分析表明,MaTPS2在香蕉植株的根、球茎、假茎、叶、花各器官中均有表达,其中在球茎、假茎和叶中表达量较多,在果实中表达量较低。在ABA、干旱、盐害和枯萎病菌胁迫处理下,MaTPS2表达量升高,在ACC胁迫处理下,MaTPS2表达量显著下降,而低温胁迫条件下,MaTPS2表达不响应。由此推导,MaTPS2可能参与调控香蕉抗旱、抗病机制。植物生长调节剂ACC和ABA胁迫处理说明MaTPS2的表达可能受激素蛋白间接调控。
Based on the full-length MaTPS2 gene sequence, homologous sequence alignment, cluster analysis and real-time quantitative PCR were used to investigate the function of MaTPS2 gene in banana. A 3 277 bp MaTPS2 full-length sequence was obtained from banana A genome. It contained a complete open reading frame (1 344 bp) encoding a protein of 447 amino acids. The analysis of its amino acid physical and chemical properties showed that the MaTPS2 protein belongs to the unstable hydrophobic protein and has a domain of GT1-TPS. The homology was 77.95% by homology comparison with known plant TPS amino acids. Phylogenetic tree phylogenetic analysis showed that the amino acid sequence of MaTPS2 was adjacent to the amino acid sequence of Ginkgo TPS (AAX16014.1), indicating that the genetic relationship between the two was close. Organ-specific analysis showed that MaTPS2 was expressed in all organs of banana plants such as roots, bulbs, pseudostems, leaves and flowers. Among them, MaTPS2 expressed more in corms, pseudostems and leaves, and lower in fruits. MaTPS2 expression increased under ABA, drought stress, salt stress and Fusarium oxysporum stress. Under the stress of ACC, the expression of MaTPS2 was significantly decreased, but under low temperature stress, MaTPS2 expression did not respond. Therefore, MaTPS2 may be involved in the regulation of banana drought resistance and disease resistance mechanism. Plant growth regulators ACC and ABA stress treatment that MaTPS2 expression may be indirectly regulated by hormonal proteins.