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庚酸睾酮（ＴＥ）诱导的无精子症等同于可逆性的男性不育。为了进一步研究ＴＥ对附睾精子成熟的影响，给受试者每周２００ｍｇ的ＴＥ，共注射３个月，分别对注射前，注射后１、２、３个月和恢复期的精浆蛋白质组分进行ＳＤＳ－聚丙烯酰胺凝胶电泳动态分析。注射ＴＥ一个月后精浆中的分子量为８７．１、６７．６、５０．１、３９．８和３１．６×１０３（ｋＤ）的主要蛋白成分开始下降，分别平均下降到注射ＴＥ前的６８．０％、４０．０％、４２．５％、４２．３％和３０．５％。但注射到精浆中无精子时某些精浆蛋白成分又增加，短暂的增加后又再次降低，甚至到恢复期尚未达到正常的水平，分别平均下降到注射前的４２．８％、１６．０％、２１．８％、２９．３％和３１．０％。经转移电泳和抗人转铁蛋白免疫化学分析，注射ＴＥ后第二个月增加的蛋白成分出现转铁蛋白阳性反应，特别是分子量６７．６×１０３的蛋白成分显著的阳性反应。结果表明精子功能低下与精浆蛋白成分下降时间相吻合。而当精浆中无精子时睾丸支持细胞分泌的转铁蛋白有短暂的增加，可能ＴＥ对支持细胞有兴奋作用。
Testosterone heptanoate (TE) -induced azoospermia is equivalent to reversible male infertility. In order to further study the effect of TE on the sperm maturation in the epididymis, subjects were injected with 200 mg of TE per week for a total of 3 months, respectively, before and after injection, 1, 2, 3 months and convalescent seminal plasma protein components SDS-polyacrylamide gel electrophoresis dynamic analysis. One month after the injection of TE, the main protein components with a molecular weight of 87.1, 67.6, 50.1, 39.8 and 31.6 × 103 (kD) in seminal plasma began to decline, respectively, 68.0%, 40.0%, 42.5%, 42.3% and 30.5%. However, when injected into the seminal plasma, some of the seminal plasma protein components increased again and then decreased again, even to the point of convalescence that they did not reach the normal level, dropping to an average of 42.8% and 16.8% respectively before injection. 0%, 21.8%, 29.3% and 31.0%. After transfer electrophoresis and anti-human transferrin immunochemical analysis, the protein components increased in the second month after TE injection showed a positive transferrin reaction, especially a significant positive reaction with a protein component of 67.6 × 103 molecular weight. The results show that low sperm motility and serum protein composition decline time coincide. However, when there is no sperm in the seminal plasma, there is a transient increase in transferrin secreted by the testicular support cells, and TE may be stimulated by the supportive cells.