论文部分内容阅读
目的探讨血清硅元素水平对矽肺早期诊断的意义。方法采用电感耦合等离子体质谱仪(ICP-MS)检测矽肺模型大鼠血清中硅元素含量,同时采用免疫组织化学-链霉菌抗生物素蛋白-过氧化物酶连结法观察转化生长因子-β1、肿瘤坏死因子-α在大鼠矽肺模型肺组织的表达。结果与对照组比较,大鼠染尘后血清硅元素水平在染尘第一天即见增高,峰值在16 h;其后的7 d、14 d、21 d和28 d,其含量水平亦显著高于对照组,差异有统计学意义。大鼠染尘后肺组织TGF-β1表达与对照组相比差异有统计学意义(P<0.05),表达高峰是染尘后的第7天;TNF-α表达也显著高于对照组,差异有统计学意义(P<0.05)。结论矽肺模型大鼠血清硅元素水平在染尘早期即见明显升高,且早于TGF-β1、TNF-α的表达,提示此一指标在矽肺的早期诊断中具有一定的价值和意义。
Objective To investigate the significance of serum silicon levels in the early diagnosis of silicosis. Methods The content of silicon in the serum of silicotic model rats was detected by inductively coupled plasma mass spectrometry (ICP-MS), and the expression of transforming growth factor-β1 (TGF-β1) was detected by immunohistochemical streptavidin-peroxidase Expression of Tumor Necrosis Factor - α in Lung Tissue of Rat Silicosis Model. Results Compared with the control group, the levels of serum silicon in the infected rats increased significantly on the first day after the dust exposure, with a peak value of 16 h. The levels of serum silicon were also significantly increased at 7 d, 14 d, 21 d and 28 d Higher than the control group, the difference was statistically significant. Compared with the control group, the expression of TGF-β1 in the lung tissue of the rats was significantly different (P <0.05), and the peak of expression was the 7th day after the infection with dust; the expression of TNF-α was also significantly higher than that of the control group There was statistical significance (P <0.05). Conclusion The level of serum silicon in silicosis model rats significantly increased at early stage and earlier than that of TGF-β1 and TNF-α, suggesting that this index has some value and significance in the early diagnosis of silicosis.