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目的探讨百合水溶液对麻黄素致损伤肺组织的影响。方法选出生10 d的小鼠72只,随机分为空白对照组、实验对照组和百合组3组。实验对照组和百合组采用递增剂量连续(1~5d,5~10d,11~15 d)腹腔注射(2.0、4.0、6.0 g/L)麻黄素溶液15 d(每次0.2 ml,每天两次),百合组注射麻黄素1 h后,再灌胃0.2 ml百合水溶液,实验对照组灌胃等量的生理盐水,空白对照组腹腔注射和灌胃等量的生理盐水。用比色法检测肺组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)活性及血浆髓过氧化物酶(MPO)活性的变化,用石蜡包埋法和HE染色法观察小鼠肺组织结构的病理变化,采用免疫组织化学法检测小鼠肺组织Bax、核因子-κB(NF-κB)蛋白表达的变化。结果实验对照组肺组织SOD、CAT和GSH-Px活性显著降低(P<0.01或P<0.05),血浆MPO活性显著升高(P<0.01或P<0.05),肺组织出现明显病理性损伤,肺泡上皮细胞局部降解、脱落,肺泡直径和肺泡隔厚度增大(P<0.01),肺组织Bax、NF-κB蛋白的阳性表达明显增强(P<0.01或P<0.05)。与实验对照组相比较,百合组肺组织SOD、CAT和GSH-Px活性升高(P<0.01或P<0.05),血浆MPO活性显著降低(P<0.05),肺组织损伤明显改善,肺泡上皮脱落减少,肺泡直径和肺泡隔厚度均减小(P<0.01或P<0.05),肺组织Bax、NF-κB蛋白的阳性表达明显减弱(P<0.01或P<0.05)。结论百合水溶液能增强机体抗氧化能力,提高机体免疫力,抑制肺组织Bax、NF-κB蛋白的阳性表达和肺组织细胞凋亡,对麻黄素致损伤肺组织有一定的保护作用。
Objective To investigate the effect of aqueous Lily solution on ephedrine-induced lung injury. Methods A total of 72 mice born for 10 days were randomly divided into blank control group, experimental control group and Lily group. The experimental control group and the lily group were treated with intraperitoneal injections of ephedrine (2.0, 4.0, and 6.0 g/L) for 15 days in increments (1 to 5 days, 5 to 10 days, 11 to 15 days) (0.2 ml each time, twice daily ) After 1 h of injection of ephedrine into the lily group, 0.2 ml of aqueous solution of lily was administered by gavage. The experimental control group was given the same volume of physiological saline by gavage, and the control group was given intraperitoneal injection and the same volume of normal saline. Colorimetry was used to detect the activities of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) and the activity of plasma myeloperoxidase (MPO) in lung tissue. Change, pathological changes of lung tissue were observed by paraffin-embedding and HE staining, and the expression of Bax and nuclear factor-κB (NF-κB) protein in lung tissue of mice was detected by immunohistochemistry. Results The activities of SOD, CAT and GSH-Px in the experimental group were significantly decreased (P<0.01 or P<0.05), and the plasma MPO activity was significantly increased (P<0.01 or P<0.05). The lung tissue showed obvious pathological damage. Alveolar epithelial cells were locally degraded and shed, alveolar diameter and alveolar septum thickness were increased (P<0.01), and the positive expression of Bax and NF-κB protein in lung tissue was significantly increased (P<0.01 or P<0.05). Compared with the experimental control group, the activities of SOD, CAT and GSH-Px in the lung tissue of the lily group increased (P<0.01 or P<0.05), plasma MPO activity decreased significantly (P<0.05), lung tissue damage was significantly improved, alveolar epithelium The shedding rate decreased, alveolar diameter and alveolar septum thickness decreased (P<0.01 or P<0.05). The positive expression of Bax and NF-κB protein in lung tissue was significantly decreased (P<0.01 or P<0.05). Conclusion Lilium aqueous solution can enhance the body’s antioxidant capacity, improve the body immunity, inhibit the positive expression of Bax, NF-κB protein in the lung tissue and apoptosis of lung tissue, and protect the injured lung tissue caused by ephedrine.