论文部分内容阅读
以纤维素滤纸为基质 ,通过碱处理、环氧活化、偶联亚氨基二乙酸二钠、固定化Cu2 +后制得了大孔纤维素亲和膜。另外 ,在活化后的膜上通过共价交联覆盖上琼脂糖 ,制得了具有类似“三明治”结构且性能优良的复合亲和膜 ,装柱后分别制得固定化金属螯合亲和膜色谱柱。对两种亲和膜进行牛血清白蛋白等温吸附测定显示 ,两者的最大吸附量分别为 1 .1 7mg/cm2 和 1 .3 0mg/cm2 ,与传统的琼脂糖凝胶类介质吸附量相当 ,表明覆盖上的琼脂糖对提高吸附量有一定作用。依据实验结果 ,可初步推断普通纤维素滤纸可以制成性能优良的亲和膜色谱介质 ,有望在大规模分离纯化生物大分子方面得到应用。
Using cellulose filter paper as substrate, macroporous cellulose affinity membrane was prepared by alkali treatment, epoxy activation, coupling of iminodiacetic acid disodium and immobilized Cu2 +. In addition, agarose was coated on the activated membrane by covalent cross-linking to obtain a composite affinity membrane with similar “sandwich” structure and excellent performance, and the immobilized metal chelate affinity membrane chromatography column. The isothermal adsorption of bovine serum albumin (BSA) on the two affinity membranes showed that the maximum adsorption capacities of the two membranes were 1.17 mg / cm 2 and 1.300 mg / cm 2, respectively, which were comparable to those of traditional agarose gel , Indicating that the agarose on the cover to increase the amount of adsorption has a certain effect. Based on the experimental results, it can be preliminarily concluded that ordinary cellulose filter paper can be made into an affinity membrane chromatography medium with good performance, and it is expected to be applied in large-scale separation and purification of biological macromolecules.