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目的研究结直肠癌组织中DNA结合分化抑制蛋白1(Id-1)和血管内皮生长因子(VEGF)的表达及其与肿瘤微血管生成、临床病理指标的相关性。方法收集我院50例结直肠癌组织和30例正常结直肠组织,用免疫组化法检测Id-1和VEGF的表达及CD34标记的微血管密度(MVD)。结果 Id-1和VEGF在结直肠癌组织中阳性表达为72%,76%,显著高于癌旁正常组织的24.00%,22.00%。结直肠癌组织中MVD为16.15±2.19,明显高于正常组织4.37±2.21(P<0.01);Id-1、VEGF和MVD三者均与肿瘤浆膜浸润、TNM分期、CEA(+)、脉管浸润、淋巴结转移及肝转移相关(均P<0.01)。癌组织中,Id-1和VEGF阳性组表达MVD值显著高于阴性组(P<0.01);结直肠癌组织中,Id-1与VEGF表达呈明显正相关性。结论 Id-1、VEGF高表达能诱导肿瘤微血管的生成,与结直肠癌的浸润及血行转移有关。
Objective To investigate the expression of DNA-binding inhibitor of metalloproteinase-1 (Id-1) and vascular endothelial growth factor (VEGF) in colorectal cancer tissues and its correlation with tumor angiogenesis and clinicopathological parameters. Methods Colorectal cancer tissues of 50 cases and normal colorectal tissues of 30 cases were collected. The expression of Id-1 and VEGF and the microvessel density (MVD) of CD34 were detected by immunohistochemistry. Results The positive rates of Id-1 and VEGF in colorectal cancer tissues were 72% and 76%, respectively, which were significantly higher than those in adjacent normal tissues (24.00% and 22.00%). The MVD in colorectal cancer tissues was 16.15 ± 2.19, which was significantly higher than that in normal tissues (4.37 ± 2.21, P <0.01). The expressions of Id-1, VEGF and MVD in colorectal cancer tissues were significantly correlated with serosal invasion, TNM stage, CEA Tubular invasion, lymph node metastasis and liver metastasis (all P <0.01). The expression of Id-1 and VEGF in cancer tissue was significantly higher than that in the negative group (P <0.01). The expression of Id-1 was positively correlated with the expression of VEGF in colorectal cancer tissues. Conclusions Id-1 and VEGF overexpression can induce the formation of tumor microvessels, which is related to the invasion and hematogenous metastasis of colorectal cancer.