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目的:建立同时测定炎可宁片中芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚的含量的高效液相色谱法。方法:色谱柱Kromasil C18(4.6 mm×250 mm,5μm);流动相甲醇(A)-0.1%的磷酸水(B)溶液,采用梯度洗脱,0～10 min,75%～85%A,10～20 min,85%～90%A;流速1.0 mL.min-1,柱温25℃,检测波长254 nm。结果:芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚分别在0.016 0～0.096 0μg,0.012 0～0.072 0μg,0.016 0～0.096 0μg,0.036 4～0.218 4μg,0.015 6～0.093 6μg与峰面积呈良好的线性关系;平均回收率分别为96.6%(RSD 0.76%,n=5)、97.2%(RSD 1.35%,n=5)、96.9%(RSD 1.57%,n=5)、98.1%(RSD 1.25%,n=5)和96.2%(RSD 0.62%,n=5)。结论:本方法快速简便,专属性强,可作为炎可宁片质量控制方法之一。
Objective: To establish a HPLC method for simultaneous determination of aloe-emodin, rhein, emodin, chrysophanol and physcion in Yan Ke Ning tablets. Methods: Kromasil C18 column (4.6 mm × 250 mm, 5 μm) was used as the mobile phase. The mobile phase consisted of methanol (A) and 0.1% phosphoric acid solution (B) 10 ~ 20 min, 85% ~ 90% A; flow rate 1.0 mL.min-1, column temperature 25 ℃, detection wavelength 254 nm. Results: Aloe-emodin, rhein, emodin, chrysophanol and physcion were detected in the range of 0.016 0 to 0.096 0μg, 0.012 0 to 0.072 0μg, 0.016 0 to 0.096 0μg, 0.036 4 to 0.218 4μg, 0.015 6 to 0.093 6μg The average recovery was 96.6% (RSD 0.76%, n = 5), 97.2% (RSD 1.35%, n = 5), 96.9% (RSD 1.57%, n = 5) 98.1% (RSD 1.25%, n = 5) and 96.2% (RSD 0.62%, n = 5). Conclusion: The method is rapid, simple and specific, and can be used as one of the quality control methods of Yan Ke Ning Tablet.