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利用葡萄扇叶病毒法国分离物F1 3 (Grapenivefanleafvirus,GFLV F1 3 )移动蛋白抗体对杭州分离物 (GFLV H)移动蛋白进行Westernblot,分析表明移动蛋白在接种GFLV H 3d后的苋色藜 (Chenopodiumamaranticolor)系统叶中就可检测到 ,随着时间推移 ,其积累量逐渐升高 ,接种 1 6d后达到最高值。接种 3 2d后的病叶已经枯黄 ,但移动蛋白积累量并没有减少。超薄切片电镜观察发现 ,在感染GFLV H的昆诺藜 (C .quinoa)和苋色藜的叶肉组织薄壁细胞中 ,病毒粒子呈纵列整齐地排列在小管状结构中 ,在胞间连丝中也发现有管状结构。免疫金标记显示胶体金能定位在细胞质、细胞壁和胞间连丝上 ,在管状结构也发现有少量的金粒子。这些结果进一步说明了GFLV是通过管状结构实现细胞间移动的
Western Blotting of Hangzhou isolate (GFLV H) was carried out by using Gramvide fanleaf virus (GFLV F1 3) moving protein antibody. The results showed that the motile protein of Chenopodima maranticolor (GF) System leaves can be detected, with the passage of time, its accumulation gradually increased, reaching the highest value after 16 days of inoculation. The diseased leaves after 3 days of inoculation were yellowish, but the accumulation of mobile protein did not decrease. Ultra-thin section electron microscopy showed that in the parenchyma cells of C. quininoa and A. amabilis infected with GFLV H, the virions were arranged neatly in small tubular structures in the middle of the intercellular space Also found in the silk tubular structure. Immunogold labeling showed that colloidal gold could be located on the cytoplasm, cell wall and cell wall, and a small amount of gold particles were found in the tubular structure. These results further illustrate that GFLV is a cell structure that allows cell-to-cell movement