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逆行荧光标记已广泛用于神经联系的研究。关于荧光示踪物是否存在选择性标记以及碘化丙啶(PI)在脑内的扩散情况,尚未见报道。本文用PI侧脑室(LV)注射,对此进行了研究。用大白鼠25只,分为3组。第1组14只,LV注射0.2％PI1～10μl。第2组6只,LV注射PI前,在乳头体平面切断一侧前脑内侧束(FMP)。第3组5只,分别在LV注射荧光染料1％伊文思蓝(EB)、0.4％溴乙啶或0.25％DAPI 10μl。结果如下:LV注射PI后,均引起动物明显的行为异常。症状有躯体震颤、共济失调及眼球震颤等。脑切片内PI荧光以注射部位及脑室为中心向远处逐渐减弱,在前脑可达同侧尾壳核的最外侧部。并在隔区的隔海马核,中脑多巴胺能细胞A_(8～10)群、中缝背核、中缝正中核、中脑尾份内侧丘系内及背侧以及小脑Purkinje细胞内观察到恒定的PI标记,且均为双侧性。而其他处则未见明显标记。结合Faglu荧光组织化学显示,几乎全部中脑多巴胺能细胞均显PI
Retrograde fluorescent labeling has been used extensively in neural association studies. There have been no reports of the presence of selective markers of fluorescent tracer and the spread of propidium iodide (PI) in the brain. In this paper, PI lateral ventricle (LV) injection, which was studied. Twenty-five rats were divided into three groups. The first group of 14, LV injection of 0.2% PI1 ~ 10μl. In Group 2, 6 rabbits in front of LV injected with PI, and cut off the lateral medullary bundle (FMP) in the plane of the papillary body. Group 3 were injected with LV fluorescent dye 1% Evans blue (EB), 0.4% ethidium bromide or 0.25% DAPI 10μl respectively. The results are as follows: LV injection of PI, all caused significant behavioral abnormalities in animals. Symptoms of somatic tremor, ataxia and nystagmus. The PI fluorescence in the brain slice gradually weakened far away from the injection site and the ventricles, reaching the outermost part of the caudate putamen in the forebrain. And observed constant in the septum of hippocampus, mesencephalic dopaminergic cells A_ (8 ~ 10), dorsal raphe, meso-nucleus, medial crest and medial meridian and Purkinje cells PI markers, and are bilateral. Others did not see the obvious mark. Combined with Faglu fluorescence histochemistry showed that almost all of the midbrain dopaminergic cells were PI