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以龙眼早熟优质品种‘石硖’与浓香型晚熟新品种‘香脆’的正、反交F1代共124株为试材,利用SSR标记鉴定杂种真实性,结合高接后的始花年龄和果实香气性状对杂交群体进行遗传多样性分析。结果表明:在20对龙眼基因组SSR引物与50对荔枝表达序列标签SSR引物中,筛选出双亲多态性引物9对,多态性引物占比为12.86%,其中6对引物可鉴定出112株‘石硖’ב香脆’杂交苗中的110株真杂种,真杂种比例为98.21%;4对引物可鉴定出12株‘香脆’ב石硖’杂交苗中的11株真杂种,真杂种比例为91.67%。UPGMA聚类分析显示,121株正、反交后代的遗传变异较大,可划分为3个大类5个亚类。正交与反交后代中,短始花年龄的植株(2.5年生)比例为31.58%和28.57%,中等始花年龄的植株(3.5~4.5年生)比例为68.42%和57.14%,长始花年龄的植株(≥5.5年)比例为0和14.29%;果实香气表现出无、淡、浓的广泛变异。
A total of 124 F1 progenies of crossbred F1 hybrids with longan variety “Shijie” and “Xiang crisp” were used as test materials. SSR markers were used to verify the authenticity of the hybrids. The combination of high flowering age And fruit aroma traits on the genetic diversity of hybrids. Nineteen pairs of polymorphic primers were screened from 20 pairs of longan genome SSR primers and 50 pairs of lychee sequence tag SSR primers. The proportion of polymorphic primers was 12.86%, among which 6 pairs of primers could identify 112 The total number of true hybrids was 98.21% in 110 hybrids of ’Shijie’ × ’Crispy’ hybrids, and 11 true hybrids among the 12 ’Xiangzi’ × ’Shijie’ hybrids were identified by 4 pairs of primers , The proportion of true hybrids was 91.67%. UPGMA cluster analysis showed that 121 positive and negative crosses had large genetic variation and could be divided into 5 subtypes of three major categories. The proportion of the plants with short flowering age (2.5 years old) was 31.58% and 28.57% in the cross between Oryza sativa and Oryza sativa L., and the proportion of plants with medium flowering age (3.5-5.4.5 years) was 68.42% and 57.14% Of the plants (≥5.5 years) were 0 and 14.29%, respectively; fruit aroma showed no variation in lightness and lightness.