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目的:研究C14orf166异位表达对肿瘤细胞增殖和迁移能力的影响。方法:构建C14orf166真核表达载体并转染Hela细胞,采用RT-PCR及蛋白质印迹法技术检测基因表达,用MTT、Transwell的方法检测C14orf166蛋白在Hela细胞的异位表达对其增殖和迁移能力的影响。结果:成功构建pcDNA3.1-C14orf166真核表达载体并使其在Hela细胞中大量表达,初步研究发现重组质粒转染组与空质粒转染组相比,细胞迁移能力增强,细胞穿膜数分别为39.6±11.7和25.7±8.6,而细胞增殖能力并没明显变化。结论:获得了真核表达载体pcDNA3.1-C14orf166,初步证实C14orf166表达能促进Hela细胞的迁移,为进一步研究c14orf166的功能奠定了基础。
Objective: To study the effect of ectopic expression of C14orf166 on the proliferation and migration of tumor cells. Methods: The C14orf166 eukaryotic expression vector was constructed and transfected into Hela cells. The gene expression was detected by RT-PCR and Western blotting. The expression of C14orf166 protein in Hela cells was detected by MTT and Transwell method. influences. Results: The pcDNA3.1-C14orf166 eukaryotic expression vector was successfully constructed and expressed in Hela cells. Preliminary studies showed that compared with the blank plasmid transfected group, the cell migration ability and cell membrane permeability were significantly increased 39.6 ± 11.7 and 25.7 ± 8.6, while cell proliferation did not change significantly. CONCLUSION: The eukaryotic expression vector pcDNA3.1-C14orf166 was obtained. It was preliminary confirmed that the expression of C14orf166 could promote the migration of Hela cells, which laid the foundation for further study on the function of c14orf166.