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本实验共用47只体重200—380克的大鼠,目的在于摸索自大鼠肾上腺静脉取血的方法,并观察肾上腺静脉流速与血浆中皮质固酮合量的关系。实验步骤简介如下: 时间—25分 S.C.1％盐酸吗啡,剂量2毫克1100克体重—15 I.P.1％戊巴比妥钠,剂量2毫克/100克阻断内源ACTH分泌。
In this experiment, 47 rats weighing 200-380 g were used in total. The aim was to explore the method of taking blood from the adrenal vein of rats and observe the relationship between the adrenal vein flow rate and the plasma corticosterone content. The experimental procedure is summarized as follows: Time -25 minutes S.C. 1% Morphine Hydrochloride, 2 mg dose 1100 g body weight -15 I.P.1% sodium pentobarbital, 2 mg / 100 g dose Blockade of endogenous ACTH secretion.