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目的:观察狼紫草水提物(AELO)对急性腹膜炎小鼠血清中炎性细胞因子浓度的影响。方法:采用煎煮法制备狼紫草水提物,0.6%醋酸腹腔注射建立小鼠急性腹膜炎模型。用双抗夹心ELISA法测量给药后小鼠血清中IL-1β、IL-6以及TNF-α等细胞因子水平。结果:给药6 h后,提示各AELO(10～40 g.kg-1)处理组血清中IL-1β、IL-6和TNF-α浓度较腹膜炎模型组均显著降低(P<0.05),且降低程度与AELO剂量呈显著正相关。结论:狼紫草水提物能有效减少IL-1β,IL-6以及TNF-α等炎性细胞因子的生成。
Objective: To observe the effects of aqueous extract of Anemarrhenae asphodeloides on the concentration of inflammatory cytokines in the serum of acute peritonitis mice. Methods: The water extract of Zucchini was prepared by decoction and the model of acute peritonitis was established by intraperitoneal injection of 0.6% acetic acid. The serum IL-1β, IL-6 and TNF-α and other cytokines were measured by double-antibody sandwich ELISA. Results: The levels of IL-1β, IL-6 and TNF-α in the serum of AELO (10-40 g.kg-1) treatment group were significantly lower than those of the peritonitis model group after 6 h of administration (P <0.05) There was a significant positive correlation between the reduction and AELO dose. Conclusion: Water extract of Wolfberry can effectively reduce the production of IL-1β, IL-6 and TNF-α and other inflammatory cytokines.