论文部分内容阅读
Objective: To investigate the apoptosis of dopaminergic neurons and the protective effect of nicotine in 1-methyl-4-phenyl-1, 2, 3, 6-tetrahydropyridine ( MPTP) -induced mouse model of Parkinson′s disease. Methods: The mouse model of Parkin-son’s disease were formed by MPTP ( 30 mg /kg /d×7, i.p.) ; and the loss and apoptosis of dopaminergic neurons was ob-served by Tyrosine Hydroxylase( TH) and TUNEL stains.In “Nicotime plus MPTP”group, mice were pretreated with nicotinebefore MPTP injection. The putative protective effect of nicotine was analyzed. Results: The number of TH-positive cells de-creased during MPTP treatment. Apoptotic neurons began to appear after three injections of MPTP and peaked on the 8th day.In the MPTP-intoxicated mice treated with nicotine, the loss of TH-positive cells was significantly less than that of MPTP-treated group ( 30 mg /kg /d ×7) ( P < 0.05) . Conclusion: The chronic treatment of MPTP can induce the apoptosis ofdopaminergic neurons in substantia nigra, and nicotine might have a neuroprotecitve effect on dopaminergic neurons againstMPTP toxicity.
Objective: To investigate the apoptosis of dopaminergic neurons and the protective effect of nicotine in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) -induced mouse model of Parkinson’s disease. Methods: The mouse model of Parkinson’s disease were formed by MPTP (30 mg / kg / d × 7, ip); and the loss and apoptosis of dopaminergic neurons was ob-served by Tyrosine Hydroxylase (TH) and TUNEL stains.In "Nicotime plus The putative protective effect of nicotine was analyzed. Results: The number of TH-positive cells de-creased during MPTP treatment. Apoptotic neurons began to appear after three injections of MPTP and peaked on the 8th day in the MPTP-intoxicated mice treated with nicotine, the loss of TH-positive cells was significantly less than that of the MPTP-treated group (30 mg / kg / d × 7) The chronic treatment of MPTP can induce the apoptosis of dopainergic neurons in substanti a nigra, and nicotine might have a neuroprotecitve effect on dopaminergic neurons against MPTP toxicity.