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比较了探针式和杯式超声探头对纳米颗粒的超声分散效果.用蒸馏水将纳米二氧化钛和纳米三氧化二铝配制成浓度为50.0mg/mL的混悬液,随后加入分散稳定剂(100%胎牛血清),分散液涡旋振荡2min后备用.分别采用探针式和杯式探头以20kHz,35%振幅超声波将上述2种混悬液振荡10min,用动态光散射法分析二氧化钛和三氧化二铝纳米颗粒的粒径大小及其分布情况.结果表明,杯式探头超声波振荡组在30,60,120,180min时间点的二氧化钛和三氧化二铝平均粒径均明显小于探针式探头超声波振荡组.同时,杯式探头超声波振荡组在120和180min时间点的二氧化钛和三氧化二铝颗粒粒径分布范围均窄于探针式探头超声波振荡组.结果说明杯式超声探头比探针式超声探头对纳米颗粒具有更好的分散效果.杯式探头在同一时间能处理多个小容积样本且特别适合于制备有致病危险性的样品或无菌样品.
The ultrasonic dispersing effect of the probe and the cup ultrasonic probe on the nanoparticles was compared.The nanometer titanium dioxide and the nano-alumina were prepared into 50.0mg / mL suspension with distilled water, then the dispersion stabilizer (100% Fetal bovine serum), and the dispersion was vortexed for 2 minutes before use.The probe and cup probes were used to oscillate the above two suspensions with ultrasonic waves at 20 kHz and 35% amplitude respectively for 10 min. Dynamic light scattering (DTA) The results showed that the average diameters of titanium dioxide and aluminum oxide in cup ultrasonic probe group at 30, 60, 120, 180min were significantly lower than those of probe probe ultrasonic vibration group. At the same time, the size distribution of titanium oxide and aluminum oxide particles in the cup-probe ultrasonic oscillation group at 120 and 180 min was narrower than that of the probe-type probe ultrasonic oscillation group.The results showed that the cup-type ultrasonic probe than the probe-type ultrasonic probe pairs Nanoparticles have a better dispersing effect. The cuvette probe can handle multiple small volume samples at the same time and is particularly suitable for the production of samples with risk of disease or sterile samples .