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OCT4和Nanog被公认是人ESC的自我更新调控基因,其中OCT4能够转录调控多种表面蛋白的表达,如SEMA6A。该文将人胰腺癌细胞株Panc-1、Bxpc-3、Aspc-1和Cfpac-1培养在无血清条件下,采用EGF、IGF-1和FGF-10诱导球体形成。用免疫荧光法分别检测这4种人胰腺癌细胞株及其球体细胞以及15例胰腺癌组织标本和13例正常胰腺组织标本中OCT4和Nanog的表达,结果显示,4种人胰腺癌细胞株在无血清-DF12培养基中5～10 d即可形成悬浮生长的球体。OCT4和Nanog在4种细胞株均有表达,且球体细胞中表达明显高于亲代细胞。在胰腺癌组织中仅有少量表达自我更新基因,在正常胰腺组织中微量表达。此外,还检测到Panc-1球体细胞表面高表达SEMA6A。由此可见,自我更新基因OCT4和Nanog在胰腺癌细胞中的表达和CSC有关,其表面蛋白SEMA6A作为胰腺癌干细胞表面标志物值得进一步研究。
OCT4 and Nanog are recognized as self-renewal regulatory genes of human ESC, in which OCT4 can transcriptionally regulate the expression of various surface proteins, such as SEMA6A. In this paper, human pancreatic cancer cell lines Panc-1, Bxpc-3, Aspc-1 and Cfpac-1 were cultured under serum-free conditions to induce the formation of spheres by EGF, IGF-1 and FGF-10. The expression of OCT4 and Nanog in four human pancreatic cancer cell lines and their spheroids, 15 pancreatic cancer tissues and 13 normal pancreatic tissues were detected by immunofluorescence. The results showed that the expression of OCT4 and Nanog in four human pancreatic cancer cell lines Serum-DF12 medium 5 to 10 days to form a suspension of growth of the ball. OCT4 and Nanog were expressed in all four cell lines, and the spheroid cells were significantly higher than the parental cells. In pancreatic cancer tissue only a small amount of self-renewal gene expression in normal pancreatic tissue micro-expression. In addition, SEMA6A was also highly expressed on the surface of Panc-1 spheroids. Thus, the expression of self-renewal genes OCT4 and Nanog in pancreatic cancer cells is related to CSC, and the surface protein SEMA6A is worth further study as a pancreatic cancer stem cell surface marker.