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目的用活细胞成像技术研究2,5-己二酮对中分子量神经丝蛋白(NF-M)在体外培养的背根神经节神经元轴突中的运输的影响。方法转染pPA-GFP-NFM质粒的背根神经节神经元体外培养,给予2,5-己二酮作用24 h后,荧光显微镜下定量观察GFP-NFM的转运量。结果给药4、8和16 mmol/L 2,5-HD组,NF-M在轴突中被转运的量分别为28.5±11.6%(P<0.05)、22.6±9.2%(P<0.01)和18.5±8.6%(P<0.01),而空白对照组中NF-M被转运的量为35.6±11.0%。结论 2,5-己二酮降低了NF-M的轴浆运输速率,这可能是2,5-己二酮引起中毒性周围神经病的致病机制之一。
Objective To investigate the effect of 2,5-hexanedione on the transport of medium-molecular-weight neurofilament protein (NF-M) in the axons of dorsal root ganglion neurons cultured in vitro using viable cell imaging. Methods The dorsal root ganglion neurons transfected with plasmid pPA-GFP-NFM were cultured in vitro and treated with 2,5-hexanedione for 24 h. Quantitative observation of GFP-NFM transport by fluorescence microscopy was performed. Results In the 4, 8 and 16 mmol / L 2,5-HD groups, the amount of NF-M transported in axons was 28.5 ± 11.6% (P <0.05) and 22.6 ± 9.2% (P <0.01) And 18.5 ± 8.6% (P <0.01), while the amount of NF-M transported to the blank control group was 35.6 ± 11.0%. Conclusions 2,5-Hexanedione decreases the axoplasmic transport rate of NF-M, which may be one of the mechanisms of 2,5-hexanedione-induced toxic peripheral neuropathy.