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检验松树花粉生活力的现行方法很费时间,而且可能因发芽的环境和操作人员使结果有变化。最普遍采用的技术是一种改进的悬滴法(Dillon和Zobel,1957),供花粉发芽的培养基为蒸馏水或加进一种糖类,检验的时间通常需要48～72小时。 采用几种非发芽的分析法检验花粉生活力,曾获得不同程度的成功。Ching和Ching(1976)发现花旗松、冷杉(Abies procera Rehd);及异叶铁杉(Tsuga heterophvlla(Raf.)Sarg.)经贮存的气干花粉滤出液,其导电率和紫外吸光率(260毫微米)与花粉的离体培养发芽力呈显著负相关。 在树木改良计划中需要检验花粉确定其生活力,供控制授粉参考之用。
The current method of testing the pollen viability of pine trees can be time consuming and may result in changes in the germination environment and the operator. The most commonly used technique is an improved hanging drop method (Dillon and Zobel, 1957). The culture medium for pollen germination is distilled water or a sugar is added. The time required for the test usually takes 48 to 72 hours. Several non-germinating assays were used to test pollen viability, with varying degrees of success. Ching and Ching (1976) found that the air-dried pollen filtrate from stored Douglas Fir, Abies procera Rehd, and Tsuga heterophylla (Raf.) Sarg. Showed electrical conductivity and UV absorbance Micron) and pollen in vitro culture germination force was significantly negatively correlated. Need to test pollen in tree improvement programs to determine its viability for controlling pollination.