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目的:使用Micro-CT在动物模型中评估前交叉韧带(anterior cruciate ligament,ACL)重建术后不同时间点以及不同位置的骨隧道面积.方法:选取15只新西兰兔,采用自体半腱肌肌腱移植物进行ACL重建,随机分为3组分别于术后3、6、12周处死并取材.所有样本均进行Micro-CT扫描(SkyScan1176,U.S.A),并通过图像重建,进一步测量分析股骨和胫骨的骨隧道面积.对每个隧道均选择三个不同的位置(入口处、中间处、出口处)进行测量,每个位置重复测量3次.比较不同术后时间和不同位置的骨道面积是否具有差异.结果:1)术后股骨隧道平均面积随时间变化不明显(3周时4.84 mm2,6周时4.57mm2,12周时4.46 mm2,P=0.99);股骨隧道入口处(近关节面处)面积相比其他位置更大,中间处面积最小:术后6周时,隧道入口处与出口处面积相比隧道中间处面积差异均具有统计学意义(P=0.0011,P=0.0106),术后12周时仅入口处与中间处的骨道面积差异具有统计学意义(P=0.0227);不同位置的股骨隧道面积随时间变化趋势不同.2)胫骨隧道平均面积在术后6周时出现明显增加(P=0.009),12周时又明显下降(p=0.0005)(3周时4.240 mm2,6周时6.577 mm2,12周时3.103 mm2);不同位置的胫骨隧道面积差异在各个时间点上均未显示出统计学意义(P＜0.05);胫骨隧道各个位置的骨道面积均随时间呈现出明显而相同的变化趋势:6周时骨道面积最大,12周时下降.结论:ACL重建术后,骨隧道面积可随时间推移而发生改变,胫骨隧道面积术后呈先增大后减小的趋势.同时,术后相同时期,同一骨隧道不同位置的骨道面积存在差异,以隧道入口处面积扩大最为明显.不同位置的骨道面积随时间的变化趋势不完全相同.采用多时间点、多位置的测量方法,可相对全面地反映前交叉韧带重建术后骨隧道面积的变化情况.“,”Objective To assess the bone tunnel area at different times and sites of the tunnel after the anterior cruciate ligament(ACL) reconstruction in rabbits using Micro-CT.Methods Fifteen rabbits were performed ACL reconstruction using semitendinosus tendon autograft and randomly allocated into 3 groups and killed at 3,6,and 12 weeks after the operation.All samples undertook the micro-CT scanning(using SkyScan 1176,Bruker,U.S.A.) and were analyzed the areas of bone tunnels of femur and tibia after the 3-demension image rebuilding.For each tunnel,the area of the entrance,middle and exit of the tunnel were measured 3 times respectively and compared.Results The average area of the femoral tunnel did not change significantly with time,being 4.84 mm2,4.57 mm2 and 4.46 mm2 at 3,6 and 12 weeks after the operation(P=0.99).At the very beginning,the femoral tunnel area at the entrance was the biggest,while that of the middle was the smallest.Six weeks after the operation,significant differences were observed between the femoral tunnel area at the entrance and middle,as well as that between the exit and middle(P=0.0011,P=0.0106);However,12 weeks after the operation,significant differences were observed only between that at the entrance and middle(P=0.0227).The average tibial tunnel area increased significantly at 6 weeks(6.577 mm2) and decreased at 12 weeks(3.103 mm2) after the operation(P=0.0005).Moreover,no significant differences were observed in the average tibial tunnel area at different time points and sites(P＜0.05).At different sites,the average tibial tunnel area expanded at 6 weeks,and then declined at 12 weeks after the operation.Conclusion The bone tunnel area changes with time after the ACL reconstruction,first increasing followed by decreasing in the average tibial tunnel area.The femur and tibial tunnel have significant differences in the tunnel area at different sites,which change differently with time.The bone tunnel expansion after the anterior cruciate ligament reconstruction can be comprehensively measured repeatedly at different sites.