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【目的】探究氮沉降增加对阔叶红松(Pinus koraiensis)混交林土壤微生物群落特征的影响。【方法】对阔叶红松林进行模拟氮沉降实验,设置对照(N0,0 kg/(hm~2·a))、低氮(N1,30 kg/(hm~2·a))、中氮(N2,60kg/(hm~2·a))和高氮(N3,120 kg/(hm~2·a))共4组处理,在实验样地内采集0~10 cm、≥10~20 cm土层中的土壤,测定土壤微生物生物量碳(SMBC)及土壤微生物生物量氮(SMBN)含量及变化。【结果】(1)模拟氮沉降未改变SMBC、SMBN及SMBC/SMBN的垂直分布;SMBC、SMBN在生长季月动态曲线均为以8月中旬为峰值的单峰型曲线,SMBC/SMBN的曲线波动较大,0~10 cm土层以N0处理的结果波动范围最小(2.83~6.97)。(2)模拟氮沉降仅对0~10 cm土层6、8月中旬的SMBC以及5、6、8月中旬的SMBC/SMBN有显著影响(P<0.05),而对SMBC、SMBN及SMBC/SMBN的生长季平均值无显著影响。【结论】模拟氮沉降对阔叶红松林土壤微生物生物量的影响仅在个别月份中表现明显,而对于整个生长季而言,更长时间的模拟氮沉降实验才可能对土壤微生物生物量产生明显的影响。
【Objective】 The objective of this study was to investigate the effects of nitrogen deposition on soil microbial community in Pinus koraiensis mixed forest. 【Method】 The experiment of simulating nitrogen deposition in broad-leaved Korean pine forest was conducted. The effects of N0,0 kg / (hm ~ 2 · a), N1,30 kg / (hm ~ 2 · a) (N2, 60kg / (hm ~ 2 · a)) and high nitrogen (N3,120 kg / (hm ~ 2 · a)) were collected in the experimental plots to collect 0 ~ 10 cm and ≥10 ~ 20 cm The content of soil microbial biomass SMBC and soil microbial biomass nitrogen (SMBN) were measured. 【Result】 (1) The simulated nitrogen deposition did not change the vertical distribution of SMBC, SMBN and SMBC / SMBN. The dynamic curves of SMBC and SMBN in the growing season were single peak curve with mid-August peak and SMBC / SMBN curve The fluctuation range of 0-10 cm soil layer with N0 is the smallest (2.83 ~ 6.97). (2) Simulated nitrogen deposition had significant effects on SMBC in mid-June and mid-August and SMBC / SMBN in mid-May and June and mid-August in 0-10 cm soil layer (P <0.05), while SMBC, SMBN and SMBC / SMBN growth season average no significant effect. 【Conclusion】 The effect of simulated N deposition on soil microbial biomass in broad-leaved Korean pine forest was only obvious in some months. However, for a longer period of simulated N deposition experiment, it was possible to produce significant soil microbial biomass Impact.