目的：探讨中国广东籍汉族人细胞色素Ｐ４５０２Ｅ１基因５′侧翼区的多态性分布规律。方法：利用聚合酶链反应和ＲｓａⅠ、ＰｓｔⅠ限制性片段长度多态性（ＰＣＲＲＦＬＰ）技术对８２名正常中国广东籍汉族人的细胞色素Ｐ４５０２Ｅ１基因５′侧翼区的多态性进行了检测。结果：８２名正常中国广东籍汉族人的细胞色素Ｐ４５０２Ｅ１基因５′侧翼区Ｃ１（ＲｓａⅠ＋，ＰｓｔⅠ）、Ｃ２（ＲｓａⅠ，ＰｓｔⅠ＋）等位基因频率分别为０８５４，０１４６；基因型Ｃ１Ｃ１、Ｃ１Ｃ２、Ｃ２Ｃ２的比率分别为０７３２、０２４４、００２４，符合ＨａｒｄｙＷｅｉｎｂｅｒｇ平衡分布。结论：中国广东籍汉族人细胞色素Ｐ４５０２Ｅ１基因５′侧翼区ＲｓａⅠ和ＰｓｔⅠ多态性分布规律与欧美人显著不同。 Objective: To investigate the distribution of 5 ’flanking region of cytochrome P4502E1 gene in Guangdong Han nationality in China. Methods: Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RsaⅠ) and restriction fragment length polymorphism (PCR-RFLP) were used to detect the polymorphisms in the 5 ’flanking region of cytochrome P4502E1 gene in 82 normal Han Chinese from Guangdong. Results: The frequencies of C1 (RsaⅠ +, PstⅠ), C2 (RsaⅠ, PstⅠ +) alleles in the 5 ’flanking region of cytochrome P4502E1 gene in 82 normal Guangdong Han Chinese were 0854 and 0146, respectively. The genotype The ratios of C1C1, C1C2 and C2C2 were 0732, 0244 and 0024, respectively, in line with the HardyWeinberg equilibrium distribution. CONCLUSION: The distribution of Rsa I and Pst I polymorphisms in the 5 ’flanking region of cytochrome P4502E1 gene in Chinese Han nationality in Guangdong Province of China is significantly different from that in Europe and the United States.