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为研究Ha－ras和Ki－ras癌基因转染和细胞对小鼠细小病毒（MVM）杀伤敏感性间的关系，两个细胞株，即Ki－ras癌基因转化细胞株DT和Ha－ras癌基因转化细胞株REF4-3，被用来作为研究材料．体外细胞集落形成率和细胞在裸小鼠体内的成瘤能力测定显示，和对照细胞NIH／3T3相比，REF4－3和DT对MVM的杀伤作用更敏感．同时，DNA杂交和蛋白免疫沉淀实验结果也显示，在REF-3和DT细胞中，无论是MVM的DNA增殖能力还是其NS－1蛋白的表达能力，均较在其对照组NIH／3T3细胞中高很多．FACA测定也显示，在感染MVM30h后，S期细胞的比例在REF4－3和DT细胞中都有增加，而在NIH／3T3细胞中却略微减少．通过PCR方法也可发现，在受到MVM抑制的REF-3肿瘤中仍可检测到MVM的DNA．
To investigate the relationship between Ha-ras and Ki-ras oncogene transfection and cell cytotoxicity against mouse parvovirus (MVM) killing, two cell lines, the Ki-ras oncogene-transformed cell line DT and Ha-ras carcinoma The gene-transformed cell line REF4-3 was used as a research material. The in vitro cell colony formation rate and the tumorigenicity of the cells in nude mice showed that REF4-3 and DT were more sensitive to MVM killing than the control cells NIH/3T3. At the same time, DNA hybridization and protein immunoprecipitation experiments also showed that both REF-3 and DT cells, both MVM’s DNA proliferative capacity and its NS-1 protein expression ability, were higher than those in its control NIH/3T3 cells. a lot of. The FACA assay also showed that after 30 h of infection with MVM, the proportion of S phase cells increased in REF4-3 and DT cells, but slightly decreased in NIH/3T3 cells. It was also found by PCR that MVM DNA could still be detected in REF-3 tumors that were inhibited by MVM.