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目的:探讨天麻素对慢性不可预见应激(CUS)大鼠抑郁样行为的改善作用及对海马脑源性神经营养因子(BDNF)及胶质原纤维酸性蛋白(GDNF)表达的影响。方法:将64只SD大鼠随机分为对照组(Sham),Sham+天麻素低、中、高剂量组,模型组(CUS),模型(CUS)+天麻素低、中、高剂量组,每组8只。对照组每天腹腔注射生理盐水(1 m L/kg),连续14天;Sham+天麻素低、中、高剂量组每天腹腔注射不同剂量的天麻素(50、100或者200 mg/kg),连续14天;模型组接受CUS造模,并且在造模结束后每天腹腔注射生理盐水(1 m L/kg),连续14天;模型+天麻素低、中、高剂量组在CUS造模结束后每天腹腔注射不同剂量的天麻素(50、100或者200 mg/kg),持续14天。随后,通过糖水偏好实验和强迫游泳实验检测各组大鼠的抑郁样行为,在行为学检测结束后处死大鼠,通过Elisa检测海马GFAP和BDNF的表达情况。结果:(1)慢性不可预见应激(CUS)可以导致明显的抑郁样行为,包括糖水偏好减少(P<0.05)和强迫游泳不动时间增加(P<0.01),CUS组大鼠海马GFAP和BDNF水平下降(P<0.01)。(2)一定剂量(100和200 mg/Kg)天麻素干预可以缓解CUS大鼠的抑郁行为,CUS与CUS+GAS(M)组(P<0.05)以及CUS与CUS+GAS(H)组之间(P<0.01)存在显著性差异。(3)中高剂量的天麻素可以恢复CUS大鼠海马的BDNF和GDNF水平,CUS与CUS+GAS(M)组(P<0.05)以及CUS与CUS+GAS(H)组之间(P<0.05)的BDNF和GDNF水平存在显著性差异。结论:天麻素可以缓解CUS模型大鼠抑郁样行为,恢复CUS模型大鼠海马的BDNF和GDNF水平。
Objective: To investigate the effect of gastrodin on the depression-like behavior and the expression of brain-derived neurotrophic factor (BDNF) and glial fibrillary acidic protein (GDNF) in rats with chronic unpredictable stress (CUS). Methods: Sixty-four Sprague-Dawley rats were randomly divided into sham, Sham + gastrodin low, medium and high dose groups, model group (CUS), model (CUS) + gastrodin low, medium and high dose groups Group of eight. The control group was given intraperitoneal injection of normal saline (1 m L / kg) daily for 14 days. Sham + gastrodin low, medium and high dose groups were injected intragastrically with different doses of gastrodin (50, 100 or 200 mg / kg) Day. The model group received CUS modeling, and injected with saline (1 m L / kg) intraperitoneally once a day for 14 consecutive days. The model + gastrodin low, medium and high dose groups were given daily Different doses of gastrodin (50, 100 or 200 mg / kg) were injected intraperitoneally for 14 days. Subsequently, the depression-like behavior of rats in each group was tested by sugar preference test and forced swimming test. Rats were sacrificed at the end of behavioral testing. The expression of GFAP and BDNF in hippocampus were detected by Elisa. Results: (1) Chronic unpredictable stress (CUS) can lead to obvious depression-like behaviors, including decreased preference for sugar (P <0.05) and increased forced immobilization time (P <0.01) BDNF levels decreased (P <0.01). (2) The intervention of gastrodin at certain dose (100 and 200 mg / Kg) could relieve the depression of CUS rats, CUS and CUS + GAS (M) group and CUS and CUS + GAS There was a significant difference between the two groups (P <0.01). (3) Gastrodin at medium and high doses could restore BDNF and GDNF in hippocampus of CUS rats, CUS and CUS + GAS (M) (P <0.05) and CUS and CUS + GAS (H) ) Of BDNF and GDNF levels there is a significant difference. Conclusion Gastrodin can relieve depression-like behavior in rats with CUS and restore BDNF and GDNF in hippocampus of CUS model rats.