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利用原位聚合法制备了樟脑分子印迹整体柱作为液相色谱固定相,通过优化制备条件和色谱条件,得到了对模板分子有较高保留性能的整体柱。采用前沿色谱分析法测定柱容量,得到有效结合位点Bt=1.815×102mmol/g和解离常数Kd=9.064 mmol/mL。Van’t Hoff方程得到的热力学参数焓值和熵值,说明了聚合物对樟脑的吸附为熵增过程。等温吸附模型证明在该聚合物表面只存在一种结合位点。
The camphor molecularly imprinted monolithic column was prepared by in-situ polymerization as the stationary phase of the liquid chromatography. The monolithic column with high retention of template molecule was obtained by optimizing the preparation conditions and chromatographic conditions. Column capacity was determined by frontier chromatographic analysis. The effective binding sites Bt = 1.815 × 102 mmol / g and dissociation constant Kd = 9.064 mmol / mL were obtained. The enthalpy and entropy of the thermodynamic parameters obtained from the Van’t Hoff equation show that the adsorption of camphor on the polymer is entropy increase process. Isothermal adsorption models demonstrate that there is only one binding site on the polymer surface.