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视黄酸(RA)能够抑制许多类型癌细胞生长、诱导细胞凋亡和调节细胞周期。本文研究了全反式视黄酸(ATRA)对人胃癌细胞的作用机理。结果表明,ATRA通过诱导细胞滞留在G_0/G_1期而显著抑制胃癌细胞生长,但ATRA不能诱导胃癌细胞凋亡;ATRA调控细胞周期与c-myc、磷酸化Rb水平的下调和p21~(WAF1/CIP1)、p53水平的上调有关,而cyclinD_1和CDK_4水平没有明显变化。在RA抗性细胞中,ATRA不能调节这些基因表达。结果证实,ATRA对胃癌细胞生长抑制与其诱导细胞滞留在G_0/G_1期有关,而与细胞凋亡的诱导无关,许多重要的、与周期相关的分子,包括cmyc、p21~(WAF1/CIP1、p53和Rb等参与细胞周期的调控。
Retinoic acid (RA) can inhibit the growth of many types of cancer cells, induce apoptosis and regulate the cell cycle. This paper studied the mechanism of action of all-trans retinoic acid (ATRA) on human gastric cancer cells. The results showed that ATRA significantly inhibited the growth of gastric cancer cells by inducing cell retention in the G_0/G_1 phase, but ATRA could not induce apoptosis of gastric cancer cells; ATRA regulated the cell cycle and the down-regulation of c-myc and phospho-Rb levels and p21~(WAF1/) CIP1) and p53 levels were up-regulated, while cyclinD_1 and CDK4 levels did not change significantly. In RA-resistant cells, ATRA cannot regulate these gene expressions. The results confirmed that the inhibition of gastric cancer cell growth by ATRA was related to its induction of cell retention in the G_0/G_1 phase, but not to the induction of apoptosis, and many important cycle-related molecules, including cmyc, p21~(WAF1/CIP1, p53). And Rb and others are involved in the regulation of cell cycle.