论文部分内容阅读
从欧洲七叶树(Aesculus hippocastanum)种子(Horse chestnut seed)中分离出的三萜烯醇部分占不皂化物的42.2%,即约为籽油的0.93%和籽的0.028%。先用0.5 mm 硅胶TLC 从种子油中分离出不皂化部分,再用醋酐-吡啶将纯化的三萜烯醇部分乙酰化。后者再经AgNO_3-硅胶(1:7)TLC(0.5 mm)进一步分段,得到丁酰鲸鱼醇(7)和5α-甘遂醇-8,23-二烯-3 β醇(9)的乙酰化纯品。用涂有OV-101液相的玻璃毛细管柱(30×0.3 mm)进行GLC 分析,程序升温(20/min,从160→300°),载气H_2(0.75 kg/m~2)。火焰离子检测器(FID),H_2 0.5 kg/m~2,空气1.4 kg/m~2,以正烷烃(C_(18)～C_(36))检测保留参数.用Varian 3700 GC-MS
The fraction of triterpene isolated from the horse chestnut seed of Aesculus hippocastanum seed accounted for 42.2% of the unsaponifiable matter, ie, about 0.93% of the seed oil and 0.028% of the seed. The unsaponifiable fraction was first separated from the seed oil by 0.5 mm silica gel TLC and the partially purified triterpene alcohol was acetylated with acetic anhydride-pyridine. The latter was further fractionated by AgNO 3 -silica gel (1: 7) TLC (0.5 mm) to give butylated hydroxytoluene (7) and 5α-glyceryl-8,23-diene-3β alcohol Acetylated pure product. GLC analysis was performed on a glass capillary column (30 × 0.3 mm) coated with OV-101 liquid phase. The temperature was programmed (20 / min from 160 → 300 °) and the carrier gas H 2 (0.75 kg / m 2). The retention parameters were detected with a flame ionization detector (FID) of 0.5 kg / m 2 H 2 and 1.4 kg / m 2 of air with n-alkanes (C 18-18 C 36)