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目的：了解湖州地区不孕不育患者支原体感染及其耐药情况，为临床治疗提供合理依据。方法对2013年1月-2013年12月在本院生殖中心就诊患者的生殖道分泌物予以支原体培养、鉴定及体外药敏试验并对结果进行统计分析。结果3279例不孕不育患者中1420例支原体培养阳性，阳性率为43.3%。单纯Uu阳性率36.0%，单纯Mh阳性率1.6%， Uu合并Mh阳性率5.6%。女性支原体阳性率高于男性，差异有统计学意义。支原体属对交沙霉素、强力霉素、美满霉素敏感性较高，对环丙沙星耐药性较高。结论本地区不孕不育患者支原体感染率较高，以Uu为主，临床治疗时必须进行相应的药物敏感试验，合理选用抗生素，以达到有效治疗的目的。“,”objective to understand the Huzhou area of infertility patients with mycoplasma infection and its drug resistance,to provide a reasonable basis for the clinical treatment.Methods: from 2013 January -2013 December to Mycoplasma in genital tract secretionspatients in our hospital reproductive center of the culture,identification and drug sensitivity test in vitro and the results were analyzed by statistical analysis.Results:3279 cases ofinfertility patients, 1420 cases of mycoplasma culture positive,the positive rate was 43.3%.Simple Uu positive rate was 36%,single Mh positive rate was 1.6%,the positive rate of Uu with Mh was 5.6%.The positive rate of Mycoplasma in female than in male,the difference has statistical significance. Mycoplasma to josamycin,doxycycline,minocycline,higher sensitivity,higherresistance to ciprofloxacin.Conclusion:the patients with higher rate of mycoplasma infection in local area of infertility,mainly in Uu and the clinical therapy must be carried out when the drug sensitive test of the corresponding,the rational use of antibiotics, in order to achieve the goal of effective treatment.