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AIM To detect the expression of caspase 3gene in primary human hepatocellular carcinoma(HCC)and investigate its relationship to p21~(WAF1)gene expression and HCC apoptosis.METHODS In situ hybridization was employedto determine caspase 3 and p21~(WAF1)expression inHCC.In situ end-labeling was used to detecthepatocytic apoptosis in HCC.RESULTS Twenty-one of 39(53.8%)cases ofHCC were found to express caspase 3transcripts,while 45.2% of HCC failed toexpress caspase 3.Non-cancerous adjacent livertissues showed more positive caspase 3(87.5%,7/8)as compared with HCC(P<0.05).The expression of caspase 3 is correlated withHCC differentiation,72.2%(13/18)ofmoderately to highly differentiated HCC showedcaspase 3 transcripts positive,while only 38.1%of poorly differentiated HCC harbored caspase 3transcripts(P<0.05).No relationship wasfound between caspase 3 expression and tumorsize or grade or metastasis,although 52.5%(5/8)of HCC with metastasis were caspase 3positive and a little higher than that with nometastasis(51.6%,P>0.05).Expression of caspase 3 alone did not affect the apoptosisindex(AI)of HCC.The AI was 7.12%o in caspase3-positive tumors(n=21),while in caspase 3-negative cases(n=18)6.59%0(P>0.05).Expression of caspase 3 clearly segregated withp21~(WAF1)positive tumors as compared withp21~(WAF1)-negative cases(16 of 23,69.6% versus5 of 16,31.3%)with statistical significance(P=0.017).In the cases with positive caspase 3and negative p21~(WAF1),the Al was found slightlyhigher,but with no statistical significance,thanthat with expression of p21~(WAF1)and caspase 3(7.21‰ vs 6.98‰,P>0.05).CONCLUSION Loss of caspase 3 expressionmay contribute to HCC carcinogenesis,althoughthe expression of caspase 3 does not correlatewell with cell apoptosis in HCC.p21~(WAF1)may bemerely one of the inhibitors which can reducecaspase 3 mediated cell apoptosis in HCCs.
AIM To detect the expression of caspase 3 gene in primary human hepatocellular carcinoma (HCC) and investigate its relationship to p21 WAF1 gene expression and HCC apoptosis. METHODS In situ hybridization was employed to determine caspase 3 and p21 WAF1 expression in HCC. In situ end-labeling was used to detect hepatocytic apoptosis in HCC.RESULTS Twenty-one of 39 (53.8%) cases ofHCC were found to express caspase 3 transcripts, while 45.2% of HCC failed to express caspase 3.Non-cancerous adjacent livertissues showed more positive Caspase 3 (87.5%, 7/8) was compared with HCC (P <0.05). The expression of caspase 3 was correlated with HCC differentiation, 72.2% (13/18) of moderated to highly differentiated HCC showed caspase 3 transcripts positive, while only 38.1 % of poorly differentiated HCC harbored caspase 3 transcripts (P <0.05) .No relationship was found between caspase 3 expression and tumorsize or grade or metastasis, although 52.5% (5/8) of HCC with metastasis were caspase 3 positive and a little higher than that with nometastasis (51.6%, P> 0.05) .Expression of caspase 3 alone did not affect the apoptosis index (AI) of HCC.The AI was 7.12% o in caspase3-positive tumors (n = 21), while in caspase 3-negative cases (n = 18) 6.59% 0 (P> 0.05) .Expression of caspase 3 clearly segregated withp21 WAF1 positive tumors as compared withp21 WAF1negative cases 16 of 23,69.6% versus5 of 16,31.3 %) with statistical significance (P = 0.017) .In the cases with positive caspase 3 and negative p21 ~ (WAF1), the Al was found slightlyhigher, but with no statistical significance, thanthat with expression of p21 ~ (WAF1) and caspase3 7.21 ‰ vs 6.98 ‰, P> 0.05) .CONCLUSION Loss of caspase 3 expression contribute to HCC carcinogenesis, although the expression of caspase 3 does not correlatewell with cell apoptosis in HCC.p21 ~ (WAF1) may be one one one of the inhibitors which can reduce caspase 3 mediated cell apoptosis in HCCs.