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为了研究土生空团菌的遗传多样性,对来自中国、美国、瑞士和法国等菌株的rDNA ITS区进行序列分析,利用Popgene32和phylip软件进行数据计算和聚类分析。序列分析表明土生空团菌rDNA ITS区的序列长度为422–447bp,遗传距离在0.000–0.051之间。居群结构和聚类分析结果表明:(1)土生空团菌有一定的遗传多样性,且遗传差异主要来自于居群内;(2)基因流Nm>1,遗传漂变不是导致土生空团菌居群遗传分化的主要因素;(3)土生空团菌的遗传分化受到地理环境的影响,而与宿主来源没有明显相关性。
In order to study the genetic diversity of soil microorganisms, the rDNA ITS regions from China, the United States, Switzerland and France were sequenced. Popgene32 and phylip software were used for data analysis and cluster analysis. Sequence analysis showed that the sequence length of rDNA ITS region was 422-447bp and the genetic distance was between 0.000-0.051. The results of population structure and cluster analysis showed that: (1) There was a certain degree of genetic diversity in A. viridis and the genetic differences mainly came from the population; (2) Gene flow Nm> 1, (3) The genetic differentiation of A. viridis was influenced by the geographical environment, but not with the host source.