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目的:观察灯盏花素对大鼠主动脉平滑肌钙激活钾通道(KCa)的影响,揭示灯盏花素在分子水平的作用机制。方法:采用Wistar大鼠20只,应用膜片钳技术,研究灯盏花素对大鼠主动脉平滑肌KCa的作用。结果:细胞贴附式膜片上,应用浓度分别为5×10-4、10-3、1.85×10-3mol/L的灯盏花素后,大鼠的通道开放概率(Po)及通道电导明显增大:Po分别由用药前的0.012±0.047增加至0.427±0.364,0.609±0.312(P<0.05),0.839±0.192(P<0.01);通道电导分别由用药前的(124±59)pS增大至(174±83)pS,(199±41)pS(P<0.05),(269±85)pS(P<0.01)。结论:灯盏花素可激活KCa,从而起到舒张血管的作用。
Objective: To observe the effect of breviscapine on calcium-activated potassium channel (KCa) in rat aorta smooth muscle, and to reveal the mechanism of action of breviscapine at the molecular level. METHODS: Twenty Wistar rats were used and patch clamp technique was used to study the effect of breviscapine on KCa of rat aortic smooth muscle. RESULTS: On the cell-attached patch, the channel open probability (Po) and channel conductance were significantly increased in rat scutellarin concentrations of 5×10-4, 10-3, and 1.85×10-3 mol/L, respectively. Increased: Po increased from 0.012±0.047 before administration to 0.427±0.364, 0.609±0.312 (P<0.05), 0.839±0.192 (P<0.01); channel conductance increased from (124±59) pS before administration, respectively As large as (174 ± 83) pS, (199 ± 41) pS (P <0.05), (269 ± 85) pS (P <0.01). Conclusion: Breviscapine can activate KCa and thus play a role in relaxing blood vessels.