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背景:静脉麻醉药异丙酚可能通过抗凋亡作用保护脑缺血神经元,但是,异丙酚抗凋亡作用的研究还很少,机制尚不明确。目的:观察异丙酚对大鼠全脑缺血再灌注损伤神经元凋亡率、坏死率和凋亡相关基因蛋白表达的影响,探讨异丙酚的脑保护作用及其机制。设计:随机对照的实验研究。地点和对象:在首都医科大学附属北京神经外科研究所进行研究。成年雄性Wistar大鼠19只,随机分为缺血组(n=7)、异丙酚组(n=7)和假手术对照组(n=5)。干预:制备大鼠全脑缺血再灌注模型。异丙酚组再灌注开始后立即静脉输注异丙酚1.5mL/h,持续30min。于再灌注24h取脑,用流式细胞仪检测凋亡率,坏死率,bcl-2,Bax和p53蛋白在大鼠海马神经元中的表达情况。主要观察指标:凋亡率,坏死率,bcl-2,Bax和p53蛋白在大鼠海马神经元中的表达情况。结果:异丙酚组海马神经元的凋亡率和坏死率犤(7.01±0.79)%和(12.80±0.92)%犦较缺血组犤(10.89±0.80)%和(16.67±1.04)%犦明显降低(P<0.01)。异丙酚组Bax和p53蛋白表达犤(49.93±5.41)%和(10.34±1.65)%犦较缺血组犤(57.05±1.91)%和(13.84±0.97)%犦明显降低(P分别<0.05和0.01),而异丙酚组bcl-2蛋白表达(9.45±1.16)%较缺血组(9.69±0.94)%未见显著性差异(P>0.05)。结论:异丙酚能够降低大鼠全脑缺血再灌注神?
BACKGROUND: Propofol, a venous anesthetic, may protect neurons from cerebral ischemia by anti-apoptotic effects. However, there is little research on the anti-apoptotic effect of propofol. The mechanism is still unclear. Objective: To investigate the effect of propofol on neuronal apoptosis, necrosis and expression of apoptosis-related protein during global cerebral ischemia / reperfusion in rats and to explore the neuroprotective effect and mechanism of propofol. Design: Randomized controlled experimental study. Location and Subject: A study was conducted at the Beijing Institute of Neurosurgery affiliated to Capital Medical University. Nineteen adult male Wistar rats were randomly divided into ischemia (n = 7), propofol (n = 7) and sham-operated control groups (n = 5). Intervention: Preparation of rat global cerebral ischemia-reperfusion model. Propofol group was infused with propofol 1.5mL / h immediately after reperfusion and continued for 30min. The brain was harvested 24 hours after reperfusion, and the apoptosis rate, necrosis rate, the expression of bcl-2, Bax and p53 protein in rat hippocampal neurons were detected by flow cytometry. MAIN OUTCOME MEASURES: Apoptosis rate, necrosis rate, expression of bcl-2, Bax and p53 protein in rat hippocampal neurons. Results: The apoptosis rate and necrosis rate of hippocampal neurons in propofol group were (7.01 ± 0.79)% and (12.80 ± 0.92)% 犦 (10.89 ± 0.80)% and (16.67 ± 1.04)% 缺Significantly lower (P <0.01). Compared with ischemic group (57.05 ± 1.91)% and (13.84 ± 0.97)% 异, the expression of Bax and p53 protein in the propofol group was significantly lower than that in the ischemic group (49.93 ± 5.41% vs 10.34 ± 1.65% And 0.01). However, the expression of bcl-2 protein in propofol group (9.45 ± 1.16%) was not significantly different from that in ischemic group (9.69 ± 0.94)% (P> 0.05). Conclusion: Propofol can reduce the global cerebral ischemia-reperfusion injury in rats?