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利用同工酶电泳技术研究蒙古冰草与航道冰草种间杂交F4代11个株系的遗传差异性。结果表明:在相同或不同生育阶段,供试材料的酯酶同工酶(EST)、过氧化物酶同工酶(POD)和超氧化物岐化酶(SOD)的位点、数目和强弱均存在差异,其表型差异是11个株系间及其与亲本间在蛋白质分子水平识别的重要遗传标记;选择抽穗期、分蘖期取样分析的同工酶酶谱特征,更能体现材料间酶谱表型的遗传差异性和提高鉴定结果的准确性;以遗传距离(GD)值0.40为基准,将13个材料聚类成6类:第1类株系6、7、9、8和10,第2类株系4和5,第3类株系1、3和2,第4类株系11,第5类父本航道冰草,第6类母本蒙古冰草;该研究结果对冰草杂交后代新品系的归类选育具有理论意义。
Isozyme electrophoresis was used to study the genetic diversity of 11 F4 hybrids between Populus simonii and Interstice. The results showed that in the same or different stages of growth, the EST, POD and SOD of the tested materials were significantly higher Weak phenotypes were found to be significant genetic markers between 11 lines and their parents at the molecular level of the protein. The characteristics of the isoenzyme zymogram of sampling and analysis at heading and tillering stage were more obvious And the accuracy of the identification results was improved. Based on the genetic distance (GD) value of 0.40, 13 materials were clustered into 6 types: type 1 strains 6,7,9,8 And 10, type 2 strains 4 and 5, type 3 strains 1, 3 and 2, type 4 strain 11, type 5 paternal path grass and type 6 maternal herb Mongolian grass. This study The results have theoretical significance for the classification and breeding of new lines of hybrids.