论文部分内容阅读
近年来利用生物细胞微核率(MNR),姐妹染色单体互换率(SCE)及染色体脆性部位(CFS)对焦化工人进行检测的报道已很多,为了进一步完善和简化这一监测系统,特试用蚕豆叶尖细胞微核系统与人淋巴细胞MNR来做对比研究。材料和方法 1.蚕豆叶尖细胞微核实验 1.1.实验材料用华中师范大学生物系培植的蚕豆,先浸种,催芽,待初生根长至2cm左右,将发芽种子移入烧杯内砂培(砂经自来水冲洗,高压消毒)。在25℃左
In recent years, many reports have been reported on the detection of coking workers by using MNR, SCE and CFS. In order to further improve and simplify this monitoring system, The Vicia faba leaf tip cell micronucleus system and human lymphocyte MNR were compared to make a comparative study. MATERIALS AND METHODS 1. Micronucleus test of Vicia faba leaf tip cells. 1.1. Experimental materials The Vicia faba cultivated in the Department of Biology, Huazhong Normal University were soaked and germinated first, and then the germinated seeds were transferred to a sand culture Tap water, autoclave). At 25 ℃ left