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目的:研究大黄厚朴中大黄素、芦荟大黄素、厚朴酚、和厚朴酚4种主要成分配伍组方对急性胰腺炎(acute pancueatitis,AP)大鼠的治疗作用及其可能的机制。方法:采用均匀设计法将4种组分配成C~H 6个配伍方。采用牛磺胆酸钠逆行注射胰胆管法,建立急性胰腺炎大鼠模型。大鼠术后15 min给药,24 h腹主动脉取血,测定血清中淀粉酶,胰脂肪酶,白细胞介素-6(IL-6),IL-10,肿瘤坏死因子-α(TNF-α)含量的变化。另取胰腺、肺组织行病理切片检查,蛋白免疫印迹(Western blot)法检测肺组织中核因子-κB(NF-κB)蛋白的表达。结果:各治疗组大鼠血清淀粉酶、胰脂肪酶,IL-6,TNF-α含量与模型组比较,均有显著下调(P<0.05,P<0.01);IL-10含量亦有所下降,但仅H组统计有显著降低(P<0.01)。病理检查结果显示,模型组大鼠胰腺组织大量片状坏死,胰腺腺泡结构不清;肺泡腔明显变小,肺泡上皮出现增生,间隔显著增大;而各治疗组胰腺和肺组织病灶坏死明显有所减轻。各治疗组肺组织中NF-κB蛋白表达与模型组比均显著下调。结论:大黄厚朴4种药效成分大黄素、芦荟大黄素、厚朴酚、和厚朴酚配伍组方对大鼠急性胰腺炎有明显治疗作用,其机制可能与NF-κB蛋白调节,炎症因子介导有关。
Objective: To study the therapeutic effect and its possible mechanism of compatibility of four main components of rhubarb, magnolol, magnolol and honokiol in rats with acute pancreas necrosis (AP). Methods: The uniform design method will be 4 kinds of components assigned to C ~ H 6 compatibility side. The rat model of acute pancreatitis was established by retrograde injection of sodium taurocholate into the pancreaticobiliary duct. The rats were sacrificed at 15 min after operation and the abdominal aorta blood was drawn 24 h later. The serum levels of amylase, pancreatic lipase, interleukin-6 (IL-6), IL-10 and tumor necrosis factor- α) content changes. In addition, pancreas and lung tissue were examined by pathological examination. The expression of nuclear factor-κB (NF-κB) in lung tissue was detected by Western blot. Results: The levels of serum amylase, pancreatic lipase, IL-6 and TNF-α in serum of rats in each treatment group were significantly decreased compared with those in model group (P <0.05, P <0.01), and IL-10 content also decreased , But only H group statistics were significantly lower (P <0.01). The results of pathology showed that a large number of pancreatic tissue were necrotic in the form of flakes and the acinar structure of the pancreas was not clear in the model group. The alveolar space was obviously smaller and the alveolar epithelium appeared hyperplasia and the interval was significantly increased. However, the necrosis of pancreas and lung tissue in each group was obvious Have eased. The expression of NF-κB in the lung tissue of each treatment group was significantly lower than that in the model group. Conclusions: Emodin, aloe-emodin, honokiol and honokiol combined with four effective components of Rhubarb and Magnolia officinalis have obvious therapeutic effects on acute pancreatitis in rats. The mechanism may be related to the regulation of NF-κB and inflammation Factor-mediated.