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目的:利用白消安建立SD(Sprague-Dawley)大鼠少精子症动物模型,为治疗人类男性不育症提供实验依据。方法:本研究取6周龄左右SD大鼠随机分为对照组和实验组,实验组通过腹腔注射不同浓度白消安(15、20、30、40、60 mg/kg体重),每个浓度分单次注射和连续10次累计注射两种方式,注射后每隔10d随机取样检测精子浓度、活力、畸形率以及曲细精管内部结构各项指标,30d出现弱精少精等不育症状后,第40d后停止取样,观察白消安对大鼠精子的作用效果。结果:单次注射15-60mg/kg剂量白消安都会使实验大鼠不同程度死亡,连续10 d累计注射剂量15 mg/kg白消安可破坏大鼠睾丸组织的部分精原干细胞,连续10 d累计注射总剂量20 mg/kg是较为理想的实验浓度,可消除全部精原干细胞和大部分支持细胞,30、40、60 mg/kg可致大鼠死亡或睾丸病变充血。结论:白消安对大鼠生殖细胞有毒害作用,通过腹腔注射一定浓度白消安可建立大鼠少精子症模型,可为外源干细胞体内转分化为生殖细胞提供准确的移植时间和实验依据。
OBJECTIVE: To establish an animal model of SD (Sprague-Dawley) oligozoospermia by busulfan and provide an experimental basis for the treatment of male infertility. Methods: In this study, 6-week-old SD rats were randomly divided into control group and experimental group, the experimental group by intraperitoneal injection of different concentrations of busulfan (15,20,30,40,60 mg / kg body weight), each concentration Single injection and 10 consecutive cumulative injection of two ways, 10d after injection of random sampling and detection of sperm concentration, vitality, deformity rate and the inner structure of seminiferous tubules various indicators, 30d showed azoospermia and other sterility symptoms After the first 40 days after the sampling was stopped to observe the effect of busulfan on rat sperm. Results: A single injection of buspirole at dose of 15-60mg / kg resulted in different degrees of death in experimental rats. The cumulative injection of 15 mg / kg busulfan for 10 days continuously destroyed some of the spermatogonial stem cells in rat testis, d cumulative total injection dose of 20 mg / kg is an ideal experimental concentration, can eliminate all spermatogonial stem cells and most of the supporting cells, 30,40,60 mg / kg can cause death or testicular congestion in rats. CONCLUSION: Busulfan has a toxic effect on germ cells in rats. A rat model of oligozoospermia can be established by intraperitoneal injection of busulfan, which can provide accurate transplantation time and experimental evidence for transdifferentiation of exogenous stem cells into germ cells .