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目的:观察扶正柔肝颗粒对肝癌HepG2细胞增殖、迁移、克隆形成及凋亡的影响。方法:分别采用MTT法、划痕实验法、平板克隆形成和Annexin V/PI双染法实验,观察扶正柔肝颗粒体外对肝癌HepG2细胞增殖、迁移、克隆形成和凋亡的影响。结果:扶正柔肝作用于HepG2细胞48h后,其对细胞的半数抑制浓度(IC_(50))为(21.0±1.3)mg/mL,当用药浓度为10mg/mL时HepG2细胞没有发生迁移;用药浓度为5mg/mL时,HepG2细胞集落基本无法形成;浓度为20mg/mL时,其早期凋亡率与5-Fu组无显著性差异(P>0.05),扶正柔肝颗粒有显著抑制细胞增殖、抑制细胞迁移、抑制集落形成及诱导HepG2细胞早期凋亡的作用,且这种作用会随其给药浓度的增加、给药时间的延长而随之增强。结论:扶正柔肝颗粒可以明显抑制人肝癌HepG2细胞的增殖、集落形成和迁移,其作用呈明显的剂量-效应关系,并能显著诱导该细胞早期调亡,为肝癌治疗提供新的药物研究方向。
Objective: To observe the effects of Fuzheng Rougan granule on the proliferation, migration, colony formation and apoptosis of HepG2 hepatocellular carcinoma cells. Methods: MTT assay, scratch assay, plate clone formation assay and Annexin V / PI double staining assay were used to observe the effect of Fuzheng Rougan granule on proliferation, migration, colony formation and apoptosis of HepG2 cells. Results: The inhibitory concentration (50_ (50)) of Fuzheng Rougan on HepG2 cells was (21.0 ± 1.3) mg / mL after 48 h treatment, and no change of HepG2 cells was observed when HepG2 cells were treated with Fuzheng Rougan at a concentration of 10 mg / The concentration of 5mg / mL, HepG2 cell colonies basically can not be formed; the concentration of 20mg / mL, the early apoptotic rate and 5-Fu group no significant difference (P> 0.05), Fuzheng Rougan particles significantly inhibited cell proliferation , Inhibit cell migration, inhibit colony formation and induce early apoptosis of HepG2 cells, and this effect will increase with the increase of its concentration and administration time. Conclusion: Fuzheng Rougan Granule can significantly inhibit the proliferation, colony formation and migration of human hepatocellular carcinoma HepG2 cells. The effect of Fuzheng Rougan Granule is obviously dose-effect relationship, and it can induce the early apoptosis of HepG2 cells and provide a new drug research direction for the treatment of liver cancer .