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不除氧条件下,在含20μL二溴烷(DBE)的10mL体系中,喹啉就能产生强而稳定的环糊精诱导室温燐光(CD-RTP)信号,最大λex/λem=276/496nm,喹啉浓度在2.0×10-6~4.0×010-4mol/L范围内与RTP信号呈良好的线性关系,检测限2.0×10-7mol/L。由于所用重原子微扰剂DBE的量很少,本发光体系仅均匀地呈轻微的雾状,而无明显沉淀,使测量精度明显改善。实验还发现,Na2SO3化学除氧可使体系RTP强度稍有增加,而通氮除氧反而因DBE的挥发损失使体系的RTP强度明显降低。
Quinoline produced a strong and stable cyclodextrin-induced CD-RTP signal in a 10 mL system containing 20 μL of dibromoalkane (DBE) without deoxygenation at a maximum of λex / λem = 276/496 nm The linear range of RTP was obtained with the concentration of quinoline in the range of 2.0 × 10-6 ~ 4.0 × 10-4 mol / L with the detection limit of 2.0 × 10-7mol / L. Due to the small amount of heavy atom perturbation agent DBE used, the light-emitting system showed a slight uniform cloudiness with no obvious precipitation, which markedly improved the measurement accuracy. The experiment also found that, Na2SO3 chemical deoxidation can make the RTP intensity of the system slightly increase, but through nitrogen deoxidation but due to the loss of DBE volatility so that the RTP intensity of the system significantly reduced.