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目的 了解春季气候变暖影响钉螺活动性及繁殖能力的作用机理。方法 春季采集的钉螺放入不同温度的恒温培养箱,模拟外界气候变暖。用酶组织化学技术观察不同温度下钉螺中枢神经节、雌雄生殖腺中的一氧化氮合酶(NOS)、琥珀酸脱氢酶(SDH)、乳酸脱氢酶(LDH)及乙酰胆碱脂酶(AchE)的变化。结果 各酶的平均灰度值随着温度升高呈下降趋势。在神经节中25℃组NOS、SDH及AchE的平均灰度值明显低于对照组(P<0.01);25℃组卵集及搴丸组织NOS、SDH及LDH的平均灰度值显著低于对照组;各实验组神经节LDH的平均灰度值与对照组相比无显著性差异(P>0.01)。结论 春季温度变化对钉螺的NOS、SDH、LDH及Ache活性均产生明显影响,温度升高使这些酶活性增强,提高了钉螺的活动性及繁殖力,为进一步研究全球气候变化对血吸虫病传播的影响提供了理论基础。
Objective To understand the mechanism of action of climate warming on snail activity and reproductive capacity in spring. Methods Snails collected in spring were placed in incubators with different temperatures to simulate the warming of the outside world. The activities of NOS, SDH, LDH and AchE in the central nervous ganglia and male and female gonads at different temperatures were observed by enzyme histochemistry. The change. Results The average gray value of each enzyme showed a downward trend with increasing temperature. The mean gray values of NOS, SDH and AchE in the ganglion at 25 ℃ were significantly lower than those in the control group (P <0.01). The average gray values of NOS, SDH and LDH in the eggs and the testis at 25 ℃ were significantly lower than those at 25 ℃ The average gray value of LDH in the ganglion of experimental group had no significant difference compared with the control group (P> 0.01). Conclusion The temperature changes in spring have a significant effect on the activity of NOS, SDH, LDH and Ache in snails. The activity of these enzymes is enhanced by the increase of temperature, which enhances the activity and fertility of snails. In order to further study the effects of global climate change on schistosomiasis transmission The impact provides the theoretical basis.